1887

Abstract

SUMMARY

Certain synthetic processes of a stable L-form and its parent streptococcus were compared to examine whether conversion was accompanied by significant alterations in the growth pattern. DNA and RNA were isolated from the streptococcus and its derived L-form, degraded, and molar base ratios measured and compared. Conversion to the L-form apparently did not result in a disturbance of synthetic processes related to DNA and RNA rates of syntheses and growth measured by extinction of cultures, colony count and dry weight increases. The stable L-form, although almost twice as slow growing, as compared with the streptococcus, retained the ability to function in an orderly manner and was capable of balanced growth. Conversion from streptococcus to L-form did not result in an addition to the DNA base complement (5-methylcytosine, 5-hydroxy-methylcytosine) nor in a quantitative alteration in the molar base ratio of either nucleic acid in the resulting L-form. The disorganization in L-form division characteristic of L-form growth was not directly related to an obvious disturbance in any of the parameters examined.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-39-1-131
1965-04-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/39/1/mic-39-1-131.html?itemId=/content/journal/micro/10.1099/00221287-39-1-131&mimeType=html&fmt=ahah

References

  1. Edwards J., Panos C. 1962; Streptococcal L-forms. V. Acid-soluble nucleotides of a group A streptococcus and derived L-form. J. Bact 84:1202
    [Google Scholar]
  2. Kandler O., Zehender C., Müller J. 1956; Vergleichende Untersuchungen über Atmungsstoffwechsel von Proteus vulgaris dessen stabiler L-Phase und den pleuro- pneumoniae-ähnlichen Organismen. Arch. Mikrobiol 24:219
    [Google Scholar]
  3. Kjeldgaard N. O., Kurland C. G. 1963; The distribution of soluble and ribosomal RNA as a function of growth rate. J. mol. Biol 6:341
    [Google Scholar]
  4. Lee K. Y., Wahl R., Barbu E. 1956; Contenu en bases puriques et pyrimidiques des acides désoxyribonucléiques des bactéries. Ann. Inst. Pasteur, Paris 91:212
    [Google Scholar]
  5. Lynn R. J., Smith P. F. 1957; Nucleic acid content of pleuropneumonialike organisms from human sources. J. Bact 74:811
    [Google Scholar]
  6. Mandel P., Sensenbrenner M. 1958; Étude comparée de l’acide ribonucléique du bacille Proteus et des formes L fixées qui en dérivent. Biochim. biophys. Acta 29:642
    [Google Scholar]
  7. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. mol. Biol 3:208
    [Google Scholar]
  8. Panos C. 1962; Streptococcal L-forms. IV. Comparison of the metabolic rates of a streptococcus and derived L-form. J. Bact 84:921
    [Google Scholar]
  9. Panos C. 1964a; Streptococcal L-forms—A biochemical synopsis. Trans. N. Y. Acad. Sci 26:954
    [Google Scholar]
  10. Panos C. 1964b; Cellular composition during growth of a streptococcus and derived L-form. Bact. Proc71
    [Google Scholar]
  11. Panos C., Barkulis S. S. 1959; Streptococcal L-forms. I. The effect of osmotic change on viability. J. Bact 78:247
    [Google Scholar]
  12. Panos C., Barkulis S. S., Hayashi J. A. 1959; Streptococcal L-forms. II. Chemical composition. J. Bact 78:863
    [Google Scholar]
  13. Panos C., Barkulis S. S., Hayashi J. A. 1960; Streptococcal L-forms. III. Effects of sonic treatment on viability. J. Bact 80:336
    [Google Scholar]
  14. Panos C., Hynes L. M. 1964; On growth and nutritional comparisons of a group A streptococcus before and after cell wall removal. Arch. Biochem. Biophys 105:326
    [Google Scholar]
  15. Schaechter M., Maaløe O., Kjeldgaard N. O. 1958; Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium J. gen. Microbiol 19:592
    [Google Scholar]
  16. Sevag M. G., Lackman D. B., Smolens J. 1938; The isolation of the components of streptococcal nucleoproteins in serologically active form. J. biol. Chem 124:425
    [Google Scholar]
  17. Sherratt H. S. A., Thomas A. J. 1953; The nucleic acid fractions of a strain of Streptococcus faecalis . J. gen. Microbiol 8:217
    [Google Scholar]
  18. Shockman G. D., Kolb J. J., Toennies G. 1957; A high speed shaker for the disruption of cells at low temperature. Biochim. biophys. Acta 24:203
    [Google Scholar]
  19. Shockman G. D., Lampen J. O. 1962; Inhibition by antibiotics of the growth of bacterial and yeast protoplasts. J. Bact 84:508
    [Google Scholar]
  20. Vendrely R., Tulasne R. 1953; Chemical constitution of the L forms of bacteria. Nature, Lond 171:262
    [Google Scholar]
  21. Weibull C., Beckman H. 1960; Growth of bacterial L forms and bacterial protoplasts. J. Bact 79:638
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-39-1-131
Loading
/content/journal/micro/10.1099/00221287-39-1-131
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error