1887

Abstract

SUMMARY: Several antibiotics were tested against a range of actinomycetes, bacteria and fungi representing types found in soil. From these tests four antibiotics, nystatin (50 μg./ml.), actidione (50 μg./ml.), polymyxin B sulphate (5·0 μg./ml.) and sodium penicillin (1·0 μg./ml.), were selected for incorporation into a starch + casein medium to achieve selective growth of actinomycetes on soil dilution plates. This mixture of antibiotics was tested with a number of soils and its efficiency compared with several other methods for selective development of actinomycete colonies. The most suitable mixture for the enumeration of soil actinomycete colonies was starch + casein medium with the two antifungal antibiotics (nystatin, actidione); for isolation of actinomycete colonies the same medium with all four antibiotics was the most satisfactory.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-38-2-251
1965-02-01
2021-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/38/2/mic-38-2-251.html?itemId=/content/journal/micro/10.1099/00221287-38-2-251&mimeType=html&fmt=ahah

References

  1. Agate A. D., Bhat J. V. 1963; A method for the preferential isolation of actinomycetes. Antonie van Leeuwenhoek 29:297
    [Google Scholar]
  2. Corbaz R., Gregory P. H., Lacey M. E. 1963; Thermophilic and mesophilicactinomycetes in mouldy hay. J. gen. Microbiol. 32:449
    [Google Scholar]
  3. Corke C. T., Chase F. E. 1956; The selective enumeration of actinomycetes in the presence of large numbers of fungi. Can. J. Microbiol. 2:12
    [Google Scholar]
  4. Corke C. T., Chase F. E. 1964; Comparative studies of actinomycete populations in acid podzolic and neutral mull forest soils. Soil Sci. Soc. Amer. Proc. 28:68
    [Google Scholar]
  5. Crook P., Carpenter C. C., Klens P. F. 1950; The use of sodium propionate in isolating actinomycetes from soils. Science 112:656
    [Google Scholar]
  6. Dulaney E.L., Larsen A. H., Stapley E. O. 1955; A note on the isolation of microorganisms from natural sources. Mycologia 47:420
    [Google Scholar]
  7. El-Nakeeb M. A., Lechevalier H. A. 1963; Selective isolation of aerobic actinomycetes. Appl. Microbiol. 11:75
    [Google Scholar]
  8. Johnson L. F. 1957; Effect of antibiotics on the numbers of bacteria and fungi isolated from soil by the dilution plate method. Phytopathology 47:630
    [Google Scholar]
  9. Kinsky S. C. 1962; Nystatin binding by protoplasts and a particulate fraction of Neurosporacrassa, and a basis for the selective toxicity of polyene antifungal antibiotics. Proc. nat. Acad. Sci., Wash. 48:1049
    [Google Scholar]
  10. Küster E., Williams S. T. 1964; Selection of media for isolation of streptomycetes. Nature, Lond. 202:928
    [Google Scholar]
  11. Lawrence C. H. 1956; A method of isolating actinomycetes from scabby potato tissue and soil with minimal contamination. Can. J. Bot. 34:44
    [Google Scholar]
  12. Lingappa Y., Lockwood J. L. 1961; A chitin medium for isolation, growth and maintenance of actinomycetes. Nature, Lond. 189:158
    [Google Scholar]
  13. Lingappa Y., Lockwood J. L. 1962; Chitin media for selective isolation and culture of actinomycetes. Phytopathology 52:317
    [Google Scholar]
  14. Martin J. P. 1950; The use of acid, rose bengal and streptomycin in the plate method for estimating soil fungi. Soil Sci. 69:215
    [Google Scholar]
  15. Okami Y., Hashimoto T., Suzuki M. 1959; Sensitivity of actinomycetes to antibiotics as a guide to identification. Symp. Taxonomy of Actinomycetes Tokyo, Japan: 1959:223
    [Google Scholar]
  16. Parkinson D., Williams S. T. 1961; A method for isolating fungi from soil microhabitats. Plant and Soil 13:347
    [Google Scholar]
  17. Porter J. N., Wilhelm J. J., Tresner H. D. 1960; Method for the preferential isolation of actinomycetes from soil. Appl. Microbiol. 8:174
    [Google Scholar]
  18. Řeháček Z. 1959; Isolation of actinomycetes and determination of the number of their spores in soil. Microbiology 28:220
    [Google Scholar]
  19. Tsao P. H., Leben C., Keitt G. W. 1960; An enrichment method for isolating actinomycetes that produce diffusible antifungal antibiotics. Phytopathology 50:88
    [Google Scholar]
  20. Waksman S. A. 1961; The Actinomycetes. 2. Classification, Identification and Description of Genera and Species. Baltimore: The Williams and Wilkins Co.;
    [Google Scholar]
  21. Williams S. T., Parkinson D., Burges N. A. 1965; An examination of the soil washing technique by its application to several soils. Plant db Soil.(In the Press.)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-38-2-251
Loading
/content/journal/micro/10.1099/00221287-38-2-251
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error