SUMMARY: The glycogen content of peptone grown cultures of the ciliate , increased from 6% cellular protein during the exponential growth phase to 30 % or more in the stationary phase of growth. The oxygen content in the medium began to decrease at a population of 7500 organisms/ml. in gently shaken cultures and at 80,000 organisms/ ml. in well aerated cultures. In either case, depletion of oxygen in the medium was soon followed by cessation of multiplication. The stationary phase was rapidly induced by transfer of log phase organisms to conditions of restricted aeration. Within 3 hr after such a transfer, the rate of glyconeogenesis approximately doubled, both in cultures and in washed suspensions of organisms incubated with 0·1 % sodium acetate.


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