1887

Abstract

SUMMARY: Erythrocytes from various species of animals were tanned by exposure to 1/20,000 tannic acid for 10 min. at 37°, and were used in various haem-agglutination systems together with untreated red cells as control. The titre of fresh human anti-AB serum was decreased from 4-to 64-fold when tested with tanned homologous red cells, while no decrease of titre was observed in commercially prepared sera of the same group under identical experimental conditions. The titre of anti-sheep red cell serum was not appreciably affected when tested with tanned sheep red cells, but there was a 2-to 4-fold increase in the agglutinin titres of myxoviruses (influenza, mumps) tested with tanned chick or sheep red cells. The haem-agglutinin titres of West Nile and Sindbis viruses were not changed when tanned goose red cells were used instead of untreated red cells in haem-agglutination tests. Human, guinea pig and fowl red cells did not become agglutinable by West Nile and Sindbis viruses after treatment with tannic acid. West Nile and Sindbis viruses agglutinated sheep red cells to low titres. These titres were twice as high when tanned red cells were used instead of untreated cells. There was a sharp drop in the titres of anti-human cell sera when tested with tanned human group O red cells. It is suggested that tannic acid may act on certain species of red cell to increase or decrease their agglutinability in the presence of immune serum.

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/content/journal/micro/10.1099/00221287-38-2-181
1965-02-01
2021-08-04
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References

  1. Boyden,S. V 1951; The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J. exp. Med. 93:107
    [Google Scholar]
  2. Brading J. 1956; The use of tannic acid to link blood group substances to group O cells. Aust. J. exp. Biol. med. Sci. 34:157
    [Google Scholar]
  3. Brand K. G., Syverton J. T. 1960; Immunology of cultivated mammalian cells. I. Species specificity determined by hemagglutination. J. nat. Cancer Inst. 24:1007
    [Google Scholar]
  4. Buckland,F. E. & Tyrrell,D. A. J 1963; A comparative study of virus haemagglutinins. The stability of haemagglutinins and red cell receptors to certain physical and chemical treatments. J. gen. Microbiol. 32:241
    [Google Scholar]
  5. Bukantz S. C., Rein C. R., Kent J. F. 1946; Studies in complement fixation. II. Preservation of sheep’s blood in citrate dextrose mixtures (modified Alsever’s solution) for use in the complement-fixation reaction. J. lab. clin. Med. 31:394
    [Google Scholar]
  6. Clarke D. H., Casals J. 1958; Techniques for hemagglutination and hemaggluti-nation-inhibition with arthropod-borne viruses. Am. J. trop. Med. Hyg. 7:561
    [Google Scholar]
  7. Garabedian G. A., Matossian R. M., Djanian A. Y. 1957; An indirect hemagglutination test for hydatid disease. J. Immun. 78:269
    [Google Scholar]
  8. Garabedian G. A., Syverton J. T. 1960; Detection of anti-HeLa antibodies in rabbit antiserum by indirect hemagglutination. Proc. Soc. exp. Biol., N.Y. 105:632
    [Google Scholar]
  9. Hornung M., Baer H. 1958; Effect of tannic acid and adsorbed purified blood group substances on human erythrocytes. Proc. Soc. exp. Biol., N.Y. 98:744
    [Google Scholar]
  10. Jensen K. E. 1956; Influenza. Diagnostic Procedures for Virus and Rickettsial Diseases, 2nd ed.. New York: APHA.;
    [Google Scholar]
  11. Moskowitz M., Calvin M. 1952; On the components and structure ofthe human red cell membrane. Exp. Cell Res. 3:33
    [Google Scholar]
  12. Neter E. 1956; Bacterial hemagglutination and hemolysis. Bad. Rev. 20:166
    [Google Scholar]
  13. Springer G. F. 1963; Enzymatic and nonenzymatie alterations of erythrocyte surface antigens. Bad. Rev. 27:191
    [Google Scholar]
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