The growth of rabbitpox virus has been studied in monolayer cultures of HeLa (ERK) cells. At 36°, virus was adsorbed to cells at about one-tenth of the theoretical collision rate. The adsorbed virus penetrated the cells exponentially, the time for half penetration being about 1 hr. New infective virus began to appear at 5 hr, and the final yield at 24 hr was about 100 pfu per cell. The synthesis of viral antigen began 1 1/2 hr after infection of the cultures; this was 1 hr before the earliest appearance of viral DNA. When virus growth was complete, over 20 separate antigens could sometimes be detected in extracts of infected cells. The yield of antigens was not reduced when virus multiplication was completely inhibited by bromodeoxyuridine. It was concluded that the synthesis of viral antigens was directed by the DNA of the infecting virus, and not by the DNA formed during virus growth.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error