1887

Abstract

A disaccharide was isolated by partial hydrolysis from the group specific polysaccharide of a streptococcus strain carrying the group antigen F. The most probable structure of the substance is 3-0-β-d-gluco-pyranosyl--acetyl-d-galactosamine. The disaccharide was 300 times more active than cellobiose in the inhibition of the F/anti-F system. This denotes that it is a component of the determinant group of the group antigen. Two other serologically active oligosaccharides were isolated from the hydrolysate. Though their structure was not fully elucidated, evidence was obtained that a tetrasaccharide consisting of two molecules of the disaccharide is an important part of a determinant group of the F antigen.

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1964-12-01
2021-07-31
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References

  1. Block R. M., Ling N. S. 1954; Devices for gradient elution in chromatography. Anal. Chem. 26:1543
    [Google Scholar]
  2. Consden R. G., Gordon H. 1948; Effect of salt on partition chromatograms. Nature, Lond. 162:180
    [Google Scholar]
  3. Crumpton M. J. 1959; Identification of amino sugars. Biochem. J. 72:479
    [Google Scholar]
  4. Dorfman A., Cifonelli J. A. 1958; In Chemistry and Biology of Mucopolysaccharides. Ciba Foundation Symposium, ed. by Wolstenhome C. E. W., O’Connor M. p. 64 London: Churchill;
    [Google Scholar]
  5. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:350
    [Google Scholar]
  6. Fuller A. T. 1938; The formamide method for the extraction of polysaccharides from haemolytic streptococci. Brit. J. exp. Path. 19:130
    [Google Scholar]
  7. Gardell S. 1953; Separation on Dowex 50 ion exchange resin of glucosamine and galactosamine and their quantitative determination. Acta chem. scand. 7:207
    [Google Scholar]
  8. Gibbons M. N. 1955; The determination of methylpentoses. Analyst 80:268
    [Google Scholar]
  9. Hugett A., St G. Nixon D. A. 1957; Use of glucose oxidase, peroxidase and O-dianisidine in determination of blood and urinary glucose. Lancet ii:368
    [Google Scholar]
  10. Jeanloz R. W., Trémège M. 1956; Color reaction of N-acetyl-amino sugar derivatives with p-dimethylbenzaldehyde. Fed. Proc. 15:282
    [Google Scholar]
  11. Kabat E. A. 1960; The upper limit for the size of the human antidextran combining site. J. Immunol. 84:82
    [Google Scholar]
  12. Kabat E. A., Mayer M. 1961 Experimental Immunochemistry Springfield, Illinois U.S.A.: Charles C. Thomas;
    [Google Scholar]
  13. MacFadyen D. A. 1945; Estimation of formaldehyde in biological mixtures. J. Mol. Chem. 158:107
    [Google Scholar]
  14. Michel M. F., Gooder H. 1962; Amino acids, amino sugars and sugars present in the cell wall of some strains of Streptococcus pyogenes. J. gen. Microbiol. 29:199
    [Google Scholar]
  15. Ottens H. 1961; Indifferente en haemolytische streptococcen met groepsantigeen F. Thesis, University of Utrecht
  16. Ottens H., Winkler K. C. 1962; Indifferent and haemolytic streptococci possessing group antigen F. J. gen. Microbiol. 28:181
    [Google Scholar]
  17. Painter T. J. 1960; Water soluble polystyrenesulphonic acid as a catalyst in the controlled fragmentation of very labile polysaccharides. Chem. Industr.1214
    [Google Scholar]
  18. Painter T. J., Morgan W. T. J. 1961; Partial acid-hydrolysis of a mucopolysaccharide without appreciable N-deacetylation of hexosamine. Nature, Lond. 191:39
    [Google Scholar]
  19. Partridge S. M. 1948; Filter-paper chromatography of sugars. Biochem. J. 42:238
    [Google Scholar]
  20. Perlin A. S. 1954; Oxidation of carbohydrate with periodate in the Warburg respirometer. J. Am. chem. Soc. 76:4101
    [Google Scholar]
  21. Reissig J. L., Strominger J. L., Leloir L. F. 1956; A modified colorimetric method for the estimation of N-acetylamino sugars. J. biol. Chem. 217:959
    [Google Scholar]
  22. Rondle C. I. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine. Biochem. J. 61:586
    [Google Scholar]
  23. Salton M. R. J. 1959; An improved method for the detection of N-acetylamino sugars on paper chromatograms. Biochim. biophys. Acta 34:308
    [Google Scholar]
  24. Schiffman G., Howe C., Kabat E. A. 1958; Immunochemical studies on blood groups. XVI. Chromatographic examination of constituents split from blood group A, B, and O (H) substances and from type XIV pneumococcal polysaccharide by Clostridium tertium enzymes J. Am. chem. Soc. 80:6662
    [Google Scholar]
  25. Schiffman G., Kabat E. A., Thompson W. 1964; Immunochemical studies on blood groups. XXX. Cleavage of A, B, and H blood-group substances by alkali. Biochemistry N.Y. 3:113
    [Google Scholar]
  26. Trevelyan W. E., Proctor D. P., Harrison I. S. 1950; Detection of sugars on paper chromatograms. Nature, Lond. 166:444
    [Google Scholar]
  27. Uchida T., Robbins P. W., Luria S. E. 1963; Analysis of the serologic determinant groups of the Salmonella E-group O-antigens. Biochemistry 2:663
    [Google Scholar]
  28. Willers J. M. N., Michel M. F., Sijsma M. J., Winkler K. C. 1964; Chemical analysis and inhibition reactions of group and type antigens of group F streptococci. J. gen. Microbiol. 36:95
    [Google Scholar]
  29. Wolfrom M. R., Juliano B. O. 1960; Chondroitin sulfate modifications. I. Carboxyl-reduced chondroitin and chondrosine J. Am. chem. Soc. 82:1673
    [Google Scholar]
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