1887

Abstract

Deoxyribonucleate (DNA) preparations from elicited genetic transformation of recipient cells, and . The frequency was low (0·0005% transformation for the most reactive of six strains), as might be expected of an interaction between two organisms as dissimilar as a rod and a coccus. Evidence that the hereditary change (attainment by susceptible cells of resistance to 500 μg. dihydrostreptomycin/ml.) was due to transformation was provided by the typical time course of the reaction, the typical linear response to decreasing concentrations of DNA below 0·1 μg./ml., and by tests of transforming activity of DNA preparations extracted from 11 dihydrostreptomycin-resistant () strains which arose by intergeneric transformation. These DNAs had relatively high transforming activity for recipient strains of both species. Thus, the region of the transforming DNA molecule from a transformant strain of was recognized and genetically integrated by populations of recipient cells at frequencies as much as 10,000 times higher than those of DNA from strains of (derived by spontaneous mutation). The results with DNA preparations from particular transformants are interpreted as indicating that the length of a DNA nucleotide sequence which is integrated by a cell during transformation may differ for different cells of the same treated population.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-37-3-369
1964-12-01
2024-12-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/37/3/mic-37-3-369.html?itemId=/content/journal/micro/10.1099/00221287-37-3-369&mimeType=html&fmt=ahah

References

  1. Bracco R. M., Krauss M. R., Roe A. S., MacLeod C. M. 1957; Transformation reactions between pneumococcus and three strains of streptococci. J. exp. Med. 106:247
    [Google Scholar]
  2. Catlin B. W. 1963; Transformation by deoxyribonucleate preparations of different average base composition. Microbial Genet. Bull. 19:5
    [Google Scholar]
  3. Catlin B. W., Cunningham L. S. 1961; Transforming activities and base contents of deoxyribonucleate preparations from various neisseriae. J. gen. Microbiol. 26:303
    [Google Scholar]
  4. Catlin B. W., Cunningham L. S. 1964a; Genetic transformation of Neisseria catarrhalis by deoxyribonucleate preparations having different average base compositions. J. gen. Microbiol. 37:341
    [Google Scholar]
  5. Catlin B. W., Cunningham L. S. 1964b; Transforming activities and base composition of deoxyribonueleates from strains of Moraxella and Mima. J. gen. Microbiol. 37:353
    [Google Scholar]
  6. Ephrussi-Taylor H. 1960; On the biological functions of deoxyribonucleic acid. Symp. Soc. gen. Microbiol. 10:132
    [Google Scholar]
  7. Ephrussi-Taylor H. 1961; Recombination analysis in microbial systems. In Growth in Living Systems. Ed. by Zarrow M. X. p. 39 New York: Basic Books;
    [Google Scholar]
  8. Goodgal S. H. 1961; Studies on transformations of Hemophilus influenzae. IV. Linked and unlinked transformations J. gen. Physiol. 45:205
    [Google Scholar]
  9. Henriksen S. D. 1952; Moraxella classification and taxonomy. J. gen. Microbiol. 6:318
    [Google Scholar]
  10. Hotchkiss R. D. 1957; Criteria for quantitative genetic transformation of bacteria. In The Chemical Basis of Heredity. Ed. by McElroy W. D., Glass B. Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  11. Hotchkiss R. D., Evans A. H. 1958; Analysis of the complex sulfonamide resistance locus of pneumococcus. Cold Spr. Harb. Symp. quant. Biol. 23:85
    [Google Scholar]
  12. Krauss M. R., MacLeod C. M. 1963; Intraspecies and interspecies transformation reactions in pneumococcus and streptococcus. J. gen. Physiol. 46:1141
    [Google Scholar]
  13. Leidy G., Hahn E., Alexander H. E. 1956; On the specificity of the desoxyribonucleic acid which induces streptomycin resistance in Hemophilus. J. exp. Med. 104:305
    [Google Scholar]
  14. Marmur J., Falkow S., Mandel M. 1963; New approaches to bacterial taxonomy. Annu. Rev. Microbiol. 17:329
    [Google Scholar]
  15. Marmur J., Schildkraut C. L., Doty P. 1961; The reversible denaturation of DNA and its use in studies of nucleic acid homologies and the biological relatedness of microorganisms. J. Chim. phys. p 945
    [Google Scholar]
  16. Marmur J., Seaman E., Levine J. 1963; Interspecific transformation in Bacillus. J. Bact. 85:461
    [Google Scholar]
  17. McCarthy B. J., Bolton E. T. 1963; An approach to the measurement of genetic relatedness among organisms. Proc. nat. Acad. Sci., Wash. 50:156
    [Google Scholar]
  18. Murray R. G. E., Truant J. P. 1954; The morphology, cell structure, and taxonomic affinities of the Moraxella. J. Bad. 67:13
    [Google Scholar]
  19. Nester E. W., Schafer M., Lederberg J. 1963; Gene linkage in DNA transfer: a cluster of genes concerned with aromatic biosynthesis in Bacillus subtilis. Genetics 48:529
    [Google Scholar]
  20. Ravin A. W. 1961; The genetics of transformation. Advanc. Genet. 10:61
    [Google Scholar]
  21. Ravin A. W., De Sa J. D. H. 1964; Genetic linkage of mutational sites affecting similar characters in pneumococcus and streptococcus. J. Bad. 87:86
    [Google Scholar]
  22. Schaeffer P. 1958; Interspecific reactions in bacterial transformation. Symp. Soc. exp. Biol. 12:60
    [Google Scholar]
  23. van Sluis C. A., Stuy J. H. 1962; On the inactivation of transforming deoxyribonucleic acid by heat. Biochem. biophys. Res. Comm. 7:213
    [Google Scholar]
  24. Young F. E., Spizizen J. 1961; Physiological and genetic factors affecting trans-formation of Bacillus subtilis. J. Bad. 81:828
    [Google Scholar]
/content/journal/micro/10.1099/00221287-37-3-369
Loading
/content/journal/micro/10.1099/00221287-37-3-369
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error