Induction and Repression of Amidase Free

Abstract

The synthesis of an inducible amidase by 8602/ was studied in cultures growing exponentially in succinate medium. Induction by both the substrate inducer acetamide, and the non-substrate inducer -acetylacetamide, was repressed by cyanoacetamide. Induction by 10m-acetylacetamide was significantly repressed by 10m-cyanoacetamide, but repression of induction by 10m-acetamide required a tenfold excess of cyanoacetamide. Amidase synthesis in a medium in which acetamide was the sole carbon + nitrogen source was also repressed by cyanoacetamide, which under these conditions inhibited the growth of non-induced bacteria. Several tricarboxylic acid cycle intermediates, and related compounds, repressed amidase synthesis in exponentially growing organisms. Catabolite repression by propionate in succinate medium was decreased by increasing the concentration of acetamide. These findings are discussed in relation to general theories of regulation of microbial enzyme synthesis.

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1964-12-01
2024-03-29
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References

  1. Attardi G., Naono S., Rouviere J., Jacob F., Gros F. 1963; Production of messenger RNA and regulation of protein synthesis. Cold Spring Harb. Symp. quant. Biol. 28:363
    [Google Scholar]
  2. Brammar W. J., Clarke P. H. 1963; Induction and repression of an enzyme in Pseudomonas aeruginosa. J. gen. Microbiol. 32:iii
    [Google Scholar]
  3. Buttin G. 1963; Mecanismes regulateurs dans la biosynth&se des enzymes du meta-bolisme du galactose chez Escherichia coli K. 12. I. La biosynthese induite de la galacto-kinase et l’induction simultanee de la sequence enzymatique J. mol. Biol. 7:164
    [Google Scholar]
  4. Clarke P. H., Meadow P. M. 1959; Evidence for the occurrence of permeases for tricarboxylic acid intermediates in Pseudomonas aeruginosa. J. gen. Microbiol. 20:144
    [Google Scholar]
  5. Cohn M., Monod J. 1953; Specific inhibition and induction of enzyme biosynthesis. Adaptation in Micro-organisms. Symp. Soc. gen. Microbiol. 7:132
    [Google Scholar]
  6. Conway E. J. 1957; Microdiffusion Analysis and Volumetric Error. , 4th ed. p 98 London: Crosby Lockwood and Son Ltd;
    [Google Scholar]
  7. Gorini L. 1963; Control by repression of a biochemical pathway. Bad. Rev. 27:182
    [Google Scholar]
  8. Jacob F., Monod J. 1961; Genetic regulatory mechanisms in the synthesis of proteins. J. mol. Biol. 3:318
    [Google Scholar]
  9. Kelly M. 1961; An investigation of the action of Pseudomonas aeruginosa on amides and related compounds. Ph.D. Thesis. University of London
  10. Kelly M., Clarke P. H. 1962; An inducible amidase produced by a strain of Pseudomonas aeruginosa. J. gen. Microbiol. 27:305
    [Google Scholar]
  11. Kelly M., Kornberg H. L. 1962a; Discontinuity of amidase formation by Pseudomonas aeruginosa. Biochim. biophys. Acta 59:517
    [Google Scholar]
  12. Kelly M., Kornberg H.L. 1962b; Amidase from Pseudomonas aeruginosa: a multi-headed enzyme. Biochim. biophys. Acta 64:190
    [Google Scholar]
  13. Kelly M., Kornberg H. L. 1964; Purification and properties of acyl transferases from Pseudomonas aeruginosa. Biockem. J. 93:557
    [Google Scholar]
  14. Kessler D. P., Rickenberg H. V. 1963; The competitive inhibition of a-methyl-glucoside uptake in Escherichia coli. Biochem. Biophys. Res. Comm. 10:482
    [Google Scholar]
  15. Loomis W. F., Magasanik B. 1964; The relation of catabolite repression to the induc-tion system for β-galactosidase in Escherichia coli. J. mol. Biol. 8:417
    [Google Scholar]
  16. Magasanik B. 1961; Catabolite repression. Cold Spring Harb. Symp. quant. Biol. 26:249
    [Google Scholar]
  17. McClatchy J. K., Rosenblum E. D. 1963; Induction of lactose utilization in Staphylococcus aureus. J. Bact. 86:1211
    [Google Scholar]
  18. McFall E., Mandelstam J. 1963; Specific metabolic repression of three induced enzymes in Escherichia coli. Biochem. J. 89:391
    [Google Scholar]
  19. Monod J., Cohn M. 1952; La biosynthese induite des enzymes (adaptation enzymatique). Advanc. Enzymol. 13:67
    [Google Scholar]
  20. Nakada D., Magasanik B. 1964; The roles of inducer and catabolite repressor in the synthesis of β-galactosidase by Escherichia coli. J. mol. Biol. 8:10
    [Google Scholar]
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