Growth in the Chick Embryo of Strains of Trachoma and Inclusion Blennorrhoea Virus of Differing Virulence Free

Abstract

SUMMARY: The growth in the chick embryo yolk sac of trachoma and inclusion conjunctivitis (TRIC) strains which differ in virulence for the chick embryo was measured in terms of ELD 50 inclusion forming units in HeLa cells and total particles. Observed differences in rates of growth are consistent with the assumption that greater virulence depends on a higher rate of multiplication in the chick embryo. All strains were equally labile when heated at 37° but only the more virulent kill chick embryos at 37°.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-37-2-277
1964-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/37/2/mic-37-2-277.html?itemId=/content/journal/micro/10.1099/00221287-37-2-277&mimeType=html&fmt=ahah

References

  1. Bedson H. S., Dumbell K. R. 1961; The effect of temperature on the growth of pox viruses in the chick embryo. J. Hyg., Camb. 59:457
    [Google Scholar]
  2. Bell S. D. Jun., Murray E. S., Carroll T. J., Snyder J. C. 1963; Studies on trachoma. III. Comparison of virus infectivity for man and chick embryos Amer. J. trop. Med. Hyg. 12:902
    [Google Scholar]
  3. Collier L. H., Sowa J. 1958; Isolation of trachoma virus in embryonate eggs. Lancet i:993
    [Google Scholar]
  4. Dubes G. R., Wenner H. A. 1957; Virulence of polioviruses in relation to variant characteristics distinguishable on cells in vitro. Virology, 4:275
    [Google Scholar]
  5. Dulbecco R., Vogt M. 1954; Plaque formation and isolation of pure lines with polio-myelitis virus. J. exp. Med. 99:2
    [Google Scholar]
  6. Furness G., Graham D. M., Reeve P. 1960; The titration of trachoma and inclusion blennorrhoea viruses in cell cultures. J. gen. Microbiol. 23:613
    [Google Scholar]
  7. Gear J. H. S., Gordon F. B., Jones B. R., Bell S. D. Jun. 1963; Nomenclature of isolates of virus from trachoma and inclusion blennorrhoea. Nature, Lond. 197:26
    [Google Scholar]
  8. Hanna L., Jawetz E., Thygeson P., Dawson C. 1960; Trachoma viruses isolated in the United States: I. Growth in embryonated eggs. Proc. Soc. exp. Biol., N.Y. 104:142
    [Google Scholar]
  9. Jawetz E., Hanna L. 1960; Trachoma viruses isolated in the United States: II. Assay of egg infectivity and stability. Proc. Soc. exp. Biol., N.Y. 105:207
    [Google Scholar]
  10. Jawetz E., Hanna L., Chino S., Zichosch J. 1962; Trachoma viruses isolated in the United States. V. Growth rates and drug inhibition patterns in embryonated eggs. Proc. Soc. exp. Biol., N.Y. 109:205
    [Google Scholar]
  11. Jones B. R. 1961; TRIC virus infections in London. Trans, ophthal. Soc. U.K. 81:367
    [Google Scholar]
  12. Jones B. R., Collier L. H., Smith C. H. 1959; Isolation of virus frominclusion blennorrhoea. Lancet, i:902
    [Google Scholar]
  13. Murray E. S., Bell S. D. Jun., Hanna A. T., Nichols R. L., Snyder J. C. 1960; Studies on trachoma. 1. Isolation and identification of strains of elementary bodies from Saudi Arabia and Egypt Amer. J. trop. Med. Hyg. 9:116
    [Google Scholar]
  14. Nizamuddin N., Dumbell K. R. 1961; A simple laboratory test to distinguish the virus of smallpox from that of alastrim. Lancet i:68
    [Google Scholar]
  15. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. Amer. J. Hyg. 27:493
    [Google Scholar]
  16. Reeve P., Taverne J. 1962; A simple method for total particle counts of trachoma and inclusion blennorrhoea viruses. Nature, Lond. 195:923
    [Google Scholar]
  17. Reeve P., Taverne J. 1963; Observations on the growth of trachoma andinclusion blennorrhoea viruses in embryonate eggs. J. Hyg., Camb. 61:67
    [Google Scholar]
  18. T’ang F. F., Chang H. L., Huang Y. T., Wang K. C. 1957; Studies on the etiology of trachoma with special reference to isolation of the virus in chick embryo. Chin. med. J 75:49
    [Google Scholar]
  19. Taverne J., Blyth W. A., Reeve P. 1964; Toxicity of the agents of trachoma and inclusion conjunctivitis. J. gen. Microbiol. 37:271
    [Google Scholar]
  20. Topley and Wilson’s Principles of Bacteriology and Immunity 1964. , 5th ed. Ed. by Wilson G. S., Miles A. A. London: Edward Arnold and Co;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-37-2-277
Loading
/content/journal/micro/10.1099/00221287-37-2-277
Loading

Data & Media loading...

Most cited Most Cited RSS feed