1887

Abstract

SUMMARY: One-step growth conditions were used in which cells began replication of poliovirus RNA almost synchronously, and in which factors limiting the rate of replication appeared to be absent. RNA of uneclipsed virus was destroyed by using light-sensitive inocula. Under these conditions, ribo-nuclease-sensitive infective RNA was detectable in unchanged amount for the first 2 hr of infection, at which time replication began abruptly; the increase of poliovirus RNA was geometrical for a further 1–2 hr.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-37-2-267
1964-11-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/37/2/mic-37-2-267.html?itemId=/content/journal/micro/10.1099/00221287-37-2-267&mimeType=html&fmt=ahah

References

  1. Cooper P. D. 1961; An improved agar cell-suspension plaque assay for poliovirus : some factors affecting efficiency of plating. Virology 13:153
    [Google Scholar]
  2. Cooper P. D. 1962; Studies on the structure and function of the poliovirion: effect of concentrated urea solutions. Virology 16:485
    [Google Scholar]
  3. Cooper P. D. 1964; Synchrony and the elimination of chance delays in the growth of poliovirus. J. gen. Microbiol 37:2
    [Google Scholar]
  4. Cooper P. D., Wilson J. N., Burta M. 1959; The bulk growth of animal cells in continuous suspension culture. J. gen. Microbiol 21:702
    [Google Scholar]
  5. Hersheya D., Dixonj Chase M. 1953; Nucleic acid economy in bacteria infected with bacteriophage T2. I. Purine and pyrimidine composition J. gen. Physiol 36:777
    [Google Scholar]
  6. Ledinkon. 1963; Genetic recombination with poliovirus type 1. Studies of crosses between a normal horse serum-resistant mutant and several guanidine resistant mutants of the same strain Virology 20:107
    [Google Scholar]
  7. Luria S. E. 1951; The frequency distribution of spontaneous bacteriophage mutants as evidence for the exponential rate of phage reproduction. Cold Spr. Harb. Symp. quant. Biol 16:463
    [Google Scholar]
  8. Montagnier L., Sanders F. K. 1963; Replicative form of encephalomyocarditis virus ribonucleic acid. Nature, Lond. 199:664
    [Google Scholar]
  9. Wilson J. N., Cooper P. D. 1962; Photodynamic demonstration of two stages in the growth of poliovirus. Virology 17:195
    [Google Scholar]
  10. Wilson J. N., Cooper P. D. 1963; Aspects of the growth of poliovirus as revealed by the photodynamic effects of neutral red and acridine orange. Virology 21:135
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-37-2-267
Loading
/content/journal/micro/10.1099/00221287-37-2-267
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error