1887

Abstract

Summary: Ten leucine-requiring mutants of were induced to revert with the alkylating agent, diethylsulphate (DES). The pattern of reversion was studied by following the appearance of leucine-independent colonies on minimal plates, and of turbid (revertant) tubes among tubes containing 10-10 treated bacteria in minimal medium. Five mutants gave high (0·6-5 x 10) frequencies of reversion. The other five gave lower (5 x 10-1·3 x 10) frequencies immediately after plating on minimal agar or dilution in minimal medium, but reversion continued to take place until a high frequency was attained. This delayed mutation was found to be independent of cell metabolism. The possibility is discussed that the different patterns of reversion reflect alkylation of different base pairs. It is suggested that mutants having guanine: cytosine (G:C) base pairs at the mutant site revert mainly by pairing errors made by alkylated guanine. Mutants with adenine: thymine (A:T) sites are assumed to revert through the production of apurinic gaps, formed by the hydrolysis of ethyladenine.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-37-1-49
1964-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/37/1/mic-37-1-49.html?itemId=/content/journal/micro/10.1099/00221287-37-1-49&mimeType=html&fmt=ahah

References

  1. Bautz E., Freese E. 1960; On the mutagenic effect of alkylating agents. Proc. nat. Acad. Sci., Wash. 46:1585
    [Google Scholar]
  2. Brooks P., Lawley P. D. 1962; The methylation of cytosine and cytidine. J. chem. Soc. p. 1348
    [Google Scholar]
  3. Freese E. 1959; On the molecular explanation of spontaneous and induced mutations. Brookhaven Symp. Biol. 12:63
    [Google Scholar]
  4. Freese E. 1963; Molecular mechanism of mutation. In Molecular Genetics. Ed. by Taylor J. H. p. 207 New York and London: Academic Press;
    [Google Scholar]
  5. Green D. M., Krieg D. R. 1961; The delayed origin of mutants induced by exposure of extracellular phage T4 to ethyl methane sulfonate. Proc. nat. Acad. Sci., Wash. 47:64
    [Google Scholar]
  6. Greer S., Zamenhof S. 1962; Studies on depurination of DNA by heat. J. molec. Biol. 4:123
    [Google Scholar]
  7. Krieg D. R. 1963a; Ethyl methane sulfonate-induced reversion of bacteriophage Trail mutants. Genetics 48:561
    [Google Scholar]
  8. Krieg D. R. 1963b; Specificity of chemical mutagenesis. In Progress in Nucleic Acid Research. Ed. by Davidson J. N., Cohn W. E. vol. 2 p. 125 New York and London: Academic Press;
    [Google Scholar]
  9. Lawley P. D., Brooks P. 1963; Further studies on the alkylation of nucleic acids and their constituent nucleotides. Biochem. J. 89:127
    [Google Scholar]
  10. Margolin P., Mukai F. 1961; The pattern of mutagen-induced back mutation in Salmonella typhimurium. Z. VererbLehre 92:330
    [Google Scholar]
  11. Pal B. C. 1962; Studies on the alkylation of purines and pyrimidines. Biochemistry 1:558
    [Google Scholar]
  12. Ryan F. J. 1955; Spontaneous mutation in nondividing bacteria. Genetics 40:726
    [Google Scholar]
  13. Ryan F. J. 1959; Bacterial mutation in the stationary phase and the question of cell turnover. J. gen. Microbiol. 21:530
    [Google Scholar]
  14. Ryan F. J., Nakada D., Schneider M. J. 1961; Is DNA replication a necessary condition for spontaneous mutation?. Z.VererbLehre 92:38
    [Google Scholar]
  15. Schwartz N. M. 1963; Nature of ethyl methane sulfonate induced reversions of lac~ mutants of Escherichia coli. Genetics 48:1357
    [Google Scholar]
  16. Strauss B., Okubo S. 1960; Protein synthesis and the induction of mutations in Escherichia coli by alkylating agents. J. Bad. 79:464
    [Google Scholar]
  17. Strelzoff E. 1962; DNA synthesis and induced mutations in the presence of 5-bromouracil. II. Induction of mutations. Z.VererbLehre 93:301
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-37-1-49
Loading
/content/journal/micro/10.1099/00221287-37-1-49
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error