1887

Abstract

Summary: The germination of spores of in media based on acid hydrolysates of casein has been studied. Germination of spores was estimated by observing the decrease in turbidity of a spore suspension and confirmed by observation with the phase contrast microscope and by the increase in permeability to stains. The optimum conditions for germination of spores suspended in phosphate buffer were incubation at 37° within the range pH 6·0-7·8 following heat shock at 85° for 10 min. Anaerobic conditions were not necessary for germination, and addition of mercaptoacetate decreased the rate of germination. The minimum requirements of compounds needed for the germination of spores of was the presence of L-α-alanine, L-phenylalanine and lactate. L-phenylalanine could be replaced by L-leucine but the rate of germination was slower. L-α-Alanine was essential for germination and was not replaced by any other amino acid examined.

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/content/journal/micro/10.1099/00221287-37-1-41
1964-10-01
2024-04-19
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References

  1. Barton-Wright E. C. 1961; Practical methods for the microbiological assay of the vitamin B complex and amino acids. Lab. Practice 10:715
    [Google Scholar]
  2. Brewer J. H. 1940; Clear liquid mediums for the aerobic cultivation of anaerobes. J. Amer. Med. Ass. 115:598
    [Google Scholar]
  3. Brooks M. E., Epps H. B. G. 1958; Taxonomic studies of the genus Clostridium: Clostridium bifermentans and C. sordellii. J. gen. Microbiol. 21:144
    [Google Scholar]
  4. Halvorson H. O., Church B. 1957; Biochemistry of spores of aerobic bacilli with special reference to germination. Bad. Rev 21:112
    [Google Scholar]
  5. Hitzman D. O., Halvorson H. O., Ukita T. 1957; Requirements for production and germination of spores of anaerobic bacteria. J. Bad. 74:1
    [Google Scholar]
  6. Levinson H. S., Sevag M. G. 1953; Stimulation of germination and respiration of spores of Bacillus megatherium by manganese and monovalent anions. J. gen. Physiol. 36:617
    [Google Scholar]
  7. Long S. K., Williams O. B. 1958; A method for removal of vegetative cells from bacterial spore preparations. J. Bad. 76:332
    [Google Scholar]
  8. Phillips A. W., Gibbs P. A. 1961; Techniques for the fractionation of microbiologically active peptides derived from casein. Biochem. J. 81:551
    [Google Scholar]
  9. Powell J. F. 1950; Factors affecting the germination of thick suspensions of Bacillus subtilis spores in L-alanine solution. J. gen. Microbiol. 4:330
    [Google Scholar]
  10. Powell J. F., Hunter J. R. 1955; Spore germination in the genus Bacillus: the modification of germination requirements as a result of preheating. J. gen. Microbiol. 13:59
    [Google Scholar]
  11. Pulvertaft R. J. V., Haynes J. A. 1951; Adenosine and spore germination; phase-contrast studies. J. gen. Microbiol. 5:657
    [Google Scholar]
  12. Smith L., de S., Douglas H. C. 1950; Factors necessary for maximum growth of Clostridium bifermentans. J. Bad. 60:9
    [Google Scholar]
  13. Thorley C. M., Wolf J. 1960; Some Germination Factors of MesophilicSpore Formers, Spores II 1961. Ed. Halvorson H. O. Minneapolis 15, Minn: Burgess Publishing Co.;
    [Google Scholar]
  14. Treadwell P. E., Jann G. J., Salle A. J. 1958; Studies on factors affecting the rapid germination of spores of Clostridium botulinum.. J. Bact. 76:549
    [Google Scholar]
  15. Walker P. D. 1963; The spore antigens of Clostridium sporogenes, Cl. bifermentans and Cl. sordellii. J. Path. Bad. 85:41
    [Google Scholar]
  16. Wynne E. S., Foster J. W. 1948; Physiological studies on spore germination with special reference to Clostridium botulinum. 3. Carbon dioxide and germination, with a note on COa and aerobic spores. J. Bad. 55:331
    [Google Scholar]
  17. Wynne E. S., Mehl D. A., Schmieding W. R. 1954; Germination of Clostridium spores in buffered glucose. J. Bact. 67:435
    [Google Scholar]
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