1887

Abstract

Summary: The fraction of bacteria of a proline-requiring auxotrophic strain of strain B which was able to originate macrocolonies on a defined nutrient medium after exposure to γ-rays under anoxic conditions was markedly increased when the organisms were deprived of proline or were treated with chloramphenicol for the initial period after irradiation. Either treatment was equally effective and the maximum degree of survival which was obtained was above that observed when the cells had been incubated throughout on a proline + inorganic salts + glucose medium. The depression in survival caused by including NaCl in the defined nutrient medium, on which the irradiated bacteria were grown, was completely eliminated by both treatments. ‘Rescue’ appears to depend on the temporary inhibition of protein synthesis.

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/content/journal/micro/10.1099/00221287-37-1-33
1964-10-01
2024-04-19
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References

  1. Adler H. I., Copeland J. C. 1962; Genetic analysis of radiation response in Escherichia coli.. Genetics 47:701
    [Google Scholar]
  2. Alper T. 1961; Variability in oxygen effect observed with micro-organisms. II. Escher-ichia coli B. Int. J. Rad. Biol 3:369
    [Google Scholar]
  3. Alper T., Gillies N. E. 1958a; Dependence of the observed oxygen effect on the post-irradiation treatment of miero-organisms. Nature, Lond. 181:961
    [Google Scholar]
  4. Alper T., Gillies N. E. 1958b; Restoration of Escherichia coli strain b after irradiation; its dependence on suboptimal growth conditions. J. gen. Microbiol 18:461
    [Google Scholar]
  5. Alper T., Gillies N. E. 1960; The relationship between growth and survival after irradiation of Escherichia coli strain b and two resistant mutants. J. gen. Microbiol. 22:113
    [Google Scholar]
  6. Alper T., Gillies N. E., Elkind M. M. 1960; The sigmoid survival curve in radio-biology. Nature, Lond. 186:1062
    [Google Scholar]
  7. Barner H. D., Cohen S. S. 1956; The relation of growth to the lethal damage induced by ultraviolet irradiation in Escherichia coli. J. Bact. 71:149
    [Google Scholar]
  8. Gale E., F.& Foulkes J. P. 1953; The assimilation of amino acids by bacteria 15: Action of antibiotics on nucleic acid and protein synthesis in Staphylococcus aurevs. Biochem. J. 53:493
    [Google Scholar]
  9. Gillies N. E. 1961; The use of auxotrophic mutants to study restoration in Escherichia coli B after ultra-violet irradiation. Int. J. Rad-. Biol. 3:379
    [Google Scholar]
  10. Gillies N. E. 1963 Reported in Radiation Effects in Physics, Chemistry and Biology. Ed. by Ebert M., Howard A. p. 182 Amsterdam: North-Holland Publishing Company;
    [Google Scholar]
  11. Gillies N. E., Alper T. 1959; Reduction in the lethal effects of radiations on Escherichia coli b by treatment with chloramphenicol. Nature, Lond. 183:237
    [Google Scholar]
  12. Howard-Flanders P., Boyce R. P., Simson E., Theriot L. 1962; A genetic locus in E. coli k12 that controls the reactivation of UV-photoproducts associated with thymine in DNA. Proc. nat. Acad. Sci., Wash. 48:2109
    [Google Scholar]
  13. Jardetzky O., Julian G. R. 1964; Chloramphenicol inhibition of polyuridylic acid binding to Escherichia coli ribosomes. Nature, Lond. 201:397
    [Google Scholar]
  14. Lederberg J. 1950; Isolation and characterisation of biochemical mutants of bacteria. Meth. Med. Res. 3:5
    [Google Scholar]
  15. Miller N., Wilkinson J. 1952; Actinometry of ionizing radiations. In Radiation Chemistry, Disc. Faraday Soc. 12: p. 50
    [Google Scholar]
  16. Okagaki H., Tsubota, &Sibatani A. 1960; Unbalanced growth and bacterial death in thymine-deficient and ultraviolet irradiated Escherichia coli. J. Bact. 80:762
    [Google Scholar]
  17. Roberts R. B., Aldous E. 1949; Recovery from ultraviolet irradiation in Escherichia coli. J. Bact. 57:363
    [Google Scholar]
  18. Wisseman C. L., Smadel J. E., Hahn F. E., Hopps H. E. 1954; Mode of action of chloramphenicol. 1. Action of chloramphenicol on assimilation of ammonia and on synthesis of proteins and nucleic acids in Escherichia coli. J. Bact. 67:662
    [Google Scholar]
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