Sedimentation Coefficients of Yeast Ribosomes Free

Abstract

Summary: The corrected sedimentation coefficients, expressed as ( ), of ribosomes from nine different yeasts, representing a wide taxonomic range, were found to be 51·5 ± 2·4, 68·6 ± 3·3 and 86·7 ± 2·9 x 10 sec. Very small quantities of a faster (approximately 128 x 10 sec.) and a slower particle were sometimes detected. Yeast ribosomes have thus sedimentation coefficients which are distinctly different from bacterial ribosomes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-37-1-153
1964-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/37/1/mic-37-1-153.html?itemId=/content/journal/micro/10.1099/00221287-37-1-153&mimeType=html&fmt=ahah

References

  1. Ashikawa J. K. 1958; Ultracentrifugal studies of microsomes from starving, non-proliferating and proliferating yeast. In Microsomal Particles and Protein Synthesis. Ed. by Roberts R. B. p. 76 New York: Pergamon Press.;
    [Google Scholar]
  2. Bowen J. T., Dagley S., Sykes J., Wild D. G. 1961; Nature of the ribosomes present in bacteria and yeast: a re-appraisal. Nature, Lond. 189:638
    [Google Scholar]
  3. Chao F. 1957; Dissociation of macromolecular ribonucleoprotein of yeast. Arch. Biochem. 70:426
    [Google Scholar]
  4. Chao F., Schachman H. K. 1956; The isolation and characterisation of a macro-molecular ribonucleoprotein from yeast. Arch. Biochem. Biophys. 61:220
    [Google Scholar]
  5. De Ley J. 1964; On the unity of bacterial ribosomes. J. gen. Microbiol. 34:219
    [Google Scholar]
  6. Koehler J. K. 1962; Variation of yeast ribosomes with nutritional state. Nature, Lond. 194:757
    [Google Scholar]
  7. Lodder J., Kreger-Van Rij N. J. W. 1952 The Yeasts p. 25 Amsterdam: North Holland Publishing Co.;
    [Google Scholar]
  8. Morgan R. S. 1962; A new form of ribosome from yeast. J. mol. Biol 4:115
    [Google Scholar]
  9. Morgan R. S., Greenspan C., Cunningham B. 1963; Dissociation of yeast ribosomes by papain. Biochim. biophys. Ada 68:642
    [Google Scholar]
  10. Schachman H. K., Pardee A. B., Stanier R. Y. 1952; Studies on macromolecular organization of microbial cells. Arch. Biochem. 38:245
    [Google Scholar]
  11. Wolfe R. G. 1956; Macromolecular constituents of proliferating and non-proliferating yeast extracts. Arch. Biochem. Biophys. 63:100
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-37-1-153
Loading
/content/journal/micro/10.1099/00221287-37-1-153
Loading

Data & Media loading...

Most cited Most Cited RSS feed