RT Journal Article SR Electronic(1) A1 Razin, S.YR 1964 T1 Factors Influencing Osmotic Fragility of Mycoplasma JF Microbiology, VO 36 IS 3 SP 451 OP 459 DO https://doi.org/10.1099/00221287-36-3-451 PB Microbiology Society, SN 1465-2080, AB SUMMARY Osmotic lysis of Mycoplasma organisms was found to depend on the temperature of incubation. The organisms were practically resistant to osmotic shock at 0°, but lysed rapidly at 37°. Their sensitivity to lysis also depended on the pH value of the suspending medium, being lowest at pH values near neutrality. Divalent and polyvalent cations, in concentrations as low as 10−5 m, protected my coplasmas from osmotic lysis. The parasitic Mycoplasma strains showed various degrees of osmotic fragility, Mycoplasma gallisepticum being the least fragile. All the parasitic strains tested were, however, more resistant to osmotic lysis than the saprophytic Mycoplasma laidlawii. The possibility that osmotic lysis of my coplasmas involves autolytic processes was tested and is discussed., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-36-3-451