1887

Abstract

SUMMARY

Mutants of resistant to oxytetracycline were produced and DNA prepared from them was then used for transforming a sensitive strain. DNA from a first-step mutant seemed to transmit a single genetic factor.

On the other hand, bacteria, transformed with DNA from a second-step mutant, showed a trimodal distribution of resistance, suggesting that at least two factors were involved in the process. Transformation with DNA from clones belonging to the first and second peaks of the distribution showed that such clones transmitted a single genetic factor for oxytetracycline resistance, while DNA from clones of the third peak transformed sensitive bacteria in a way similar to that of the second step mutant. The results suggested that the two factors are linked and that they had a cumulative effect on drug resistance.

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/content/journal/micro/10.1099/00221287-36-3-423
1964-09-01
2021-08-02
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References

  1. Cavalli L. L., Maccacaro G. 1950; Chloromycetin resistance in E. coli, a case of quantitative inheritance in bacteria. Nature; Lond: 166991
    [Google Scholar]
  2. Demerec M. 1945; Production of Staphylococcus strains resistant to various concentrations of penicillin. Proc. nat. Acad. Sci., Wash 31:16
    [Google Scholar]
  3. Demerec M. 1948; Origin of bacterial resistance to antibiotics. J. Bact 56:63
    [Google Scholar]
  4. Ephrati-Elizur E. E., Srinivasan P. R., Zamenhof S. 1961; Genetic analysis, by means of transformation, of histidine linkage groups in Bacillus subtilis. Proc. nat. Acad. Sci., Wash 47:56
    [Google Scholar]
  5. Hotchkiss R. D. 1951; Transfer of penicillin resistance in pneumococci by the desoxyribonucleate derived from resistant cultures. Cold Spr. Harb. Symp. quant. Biol 16:457
    [Google Scholar]
  6. Hotchkiss R. D., Evans A. H. 1957; Genetic and metabolic mechanisms underlying multiple levels of sulphonamide resistance in pneumococci. In Drug Resistance in Microorganisms Wolstenholme G. E. W., O’Connor C. M. Ed. by London: J. and A. Churchill Ltd;
    [Google Scholar]
  7. Hsu Yu-Chin, Herriott R. M. 1961; Studies on transformations of Hemophilus influenzae. III. The genotypes and phenotypic patterns of three streptomycin-resistant mutants. J. gen. Physiol 45:197
    [Google Scholar]
  8. Magni G. E., von Borstel R. C. 1962; Different rates of spontaneous mutation during mitosis and meiosis in yeast. Genetics 47:1097
    [Google Scholar]
  9. Nester E. W., Lederberg J. 1961; Linkage of genetic units of Bacillus subtilis in deoxyribonucleic acid (DNA) transformation. Proc. nat. Acad. Sci., Wash 47:52
    [Google Scholar]
  10. Ravin A. W., Iyer Y. N. 1961; The genetic relationship and phenotypic expression of mutations endowing pneumococcus with resistance to erythromycin. J. gen. Microbiol 26:277
    [Google Scholar]
  11. Spizizen J. 1958; Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc. nat. Acad. Sci., Wash 44:1072
    [Google Scholar]
  12. Young F. E., Spizizen J. 1961; Physiological and genetic factors affecting transformation of Bacillus subtilis. J. Bact 81:823
    [Google Scholar]
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