1887

Abstract

SUMMARY: Nuclease activity was observed in several saprophytic and parasitic Mycoplasma organisms; the nucleases of were studied in detail. Nuclease activity of this organism was highest at the early logarithmic phase of growth, and was found mainly in the soluble fraction of the organisms. Anion-exchange chromatography of the proteins of the soluble fraction separated ribonuclease from deoxyribonuclease activity. Each enzyme had an alkaline pH optimum, required magnesium ions for activity, and degraded native and heat-denatured nucleic acids. RNase degraded RNA-core. RNA inhibited the degradation of DNA by deoxyribonuclease. The implications of these findings with respect to the effects of RNA and DNA on growth of are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-36-2-323
1964-08-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/36/2/mic-36-2-323.html?itemId=/content/journal/micro/10.1099/00221287-36-2-323&mimeType=html&fmt=ahah

References

  1. Anfinsen C. B., White F. H. Jr 1961; The ribonucleases: occurrence, structure and properties. In The Enzymes, 2nd ed. Boyer P. D., Lardy H., Myrbäck K. Ed. by 5 p. 95 New York and London: Academic Press;
    [Google Scholar]
  2. Barker G. R., Cannon M. 1960; The ribonuclease activity of Micrococcus lysodeikticus . Biochem. J 75:8P
    [Google Scholar]
  3. Bernheimer A. W., Ruffier N. K. 1951; Elaboration of deoxyribonuclease by streptococci in the resting state and inhibition of the enzyme by a substance extractable from the cocci. J. exp. Med 93:399
    [Google Scholar]
  4. Catlin B. W., Cunningham L. S. 1958; Studies of extracellular and intracellular bacterial deoxyribonucleic acids. J. gen. Microbiol 19:522
    [Google Scholar]
  5. Cunningham L. 1959; Micrococcal nuclease and some products of its action. Ann. N.Y. Acad. Sci 81:788
    [Google Scholar]
  6. Eaves G. N., Jeffries C. D. 1963; Isolation and properties of an exocellular nuclease of Serratia marcescens . J. Bact 85:273
    [Google Scholar]
  7. Elson D. 1959; Latent enzymic activity of a ribonucleoprotein isolated from Escherichia coli . Biochim. biophys. Acta 36:372
    [Google Scholar]
  8. Frisch-Niggemeyer W., Reddi K. K. 1957; Studies of ribonuclease in tobacco leaves. 1. Purification and properties. Biochim. biophys. Acta 26:40
    [Google Scholar]
  9. Grunberg-Manago M. 1961; Polynucleotide phosphorylase. In The Enzymes, 2nd ed. Boyer P. D., Lardy H., Myrbäck K. Ed. by 5 p. 257 New York and London: Academic Press;
    [Google Scholar]
  10. Healy J. W., Stollar D., Simon M. I., Levine L. 1963; Characterization of a phosphodiesterase from lamb brain. Arch. Biochem. Biophys 103:461
    [Google Scholar]
  11. Jeffries C. D., Holtman D. F., Guse D. 1957; Rapid method for determining the activity of microorganisms on nucleic acids. J. Bact 73:590
    [Google Scholar]
  12. Khorana H. G. 1961; Phosphodiesterases. In The Enzymes, 2nd ed. Boyer P. D., Lardy H., Myrback K. Ed. by 5 p. 79 New York and London: Academic Press;
    [Google Scholar]
  13. Lehman I. R., Roussos G. G., Pratt E. A. 1962a; The deoxyribonucleases of Escherichia coli. II. Purification and properties of a ribonucleic acid-inhibitable endonuclease. J. biol. Chem 237:819
    [Google Scholar]
  14. Lehman I. R., Roussos G. G., Pratt E. A. 1962b; The deoxyribonucleases of Escherichia coli. III. Studies on the nature of the inhibition of endonuclease by ribonucleic acid. J. biol. Chem 237:829
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  16. Plackett P. 1957; Depolymerization of ribonucleic acid by extracts of Asterococcus mycoides . Biochim. biophys. Acta 26:664
    [Google Scholar]
  17. Razin S. 1962; Nucleic acid precursor requirements of Mycoplasma laidlawii . J. gen. Microbiol 28:243
    [Google Scholar]
  18. Razin S. 1963; Osmotic lysis of Mycoplasma. J. gen. Microbiol 33:471
    [Google Scholar]
  19. Razin S., Cohen A. 1963; Nutritional requirements and metabolism of Mycoplasma laidlawii . J. gen. Microbiol 30:141
    [Google Scholar]
  20. Razin S., Knight B. C. J. G. 1960; The effects of ribonucleic acid and deoxyribonucleic acid on the growth of Mycoplasma. J. gen. Microbiol 22:504
    [Google Scholar]
  21. Stevens A., Hilmoe R. J. 1960; Studies on a nuclease from Azotobacter agilis. I. Isolation and mode of action. J. biol. Chem 235:3016
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-36-2-323
Loading
/content/journal/micro/10.1099/00221287-36-2-323
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error