1887

Abstract

SUMMARY

Growth of was partially inhibited by 1·2 × 10 -ethidium bromide, a phenanthridinium trypanocide. In the presence of manganese the drug’s effect was decreased. During growth in the presence of ethidium, RNA and protein contents were relatively unaffected when comparison was made between experimental and control cultures at similar turbidities; DNA content, on the other hand, was considerably decreased. A differential effect of ethidium on the formation of polynucleotide pyrimidines from labelled uracil and orotic acid was observed. Oxygen uptake continued almost unchanged during growth whether in the presence or absence of drug.

was extremely sensitive to the growth-inhibitory action of ethidium (10 ) and morphological changes were observed. Manganese protected the organisms from the drug’s actions. RNA and DNA biosynthesis were both suppressed during inhibition of growth to a greater extent than was total protein formation, whereas diaminopimelic acid incorporation into cell wall and oxygen uptake continued almost unaffected. Some evidence was obtained that the pattern of protein synthesis was disturbed.

It was concluded that the drug’s actions were species dependent, and that the effect on resembled that described for a flagellate, while that on did not. Evidence for compartmentation of nucleic acid synthesis, as obtained with the drug in tumour cells, was not shown for either micro-organism.

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1964-08-01
2024-03-28
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References

  1. Alpen E. L., Mandel H. G. 1960; A rapid assay method for tritium in bacterial cells. Biochim. biophys. Acta 43:317
    [Google Scholar]
  2. Brownlee G., Goss M. D., Goodwin L. G., Woodbine M., Walls L. P. 1950; The chemotherapeutic action of phenanthridine compounds. Part. I. T. congolense and T. rhodesiense. Br. J. Pharmac. Chemother 5:261
    [Google Scholar]
  3. Burton K. 1955; The relation between the synthesis of DNA and synthesis of protein in the multiplication of bacteriophage T-2. Biochem. J 61:473
    [Google Scholar]
  4. Elliott W. H. 1963; The effects of antimicrobial agents on deoxyribonucleic acid polymerase. Biochem. J 86:562
    [Google Scholar]
  5. Kandaswamy T. S., Henderson J. F. 1962; Intracellular differentiation of purine ribonucleotides derived from endogenous and exogenous sources. Biochim. biophys. Acta 61:86
    [Google Scholar]
  6. Kandaswamy T. S., Henderson J. F. 1963; Alteration of the biochemical effects of ethidium bromide by exposure of cells to hypotonic media. Nature; Lond: 199807
    [Google Scholar]
  7. Kerridge D. 1958; The effect of actidione and other antifungal agents on nucleic acid and protein synthesis in Saccharomyces carlsbergensis. J. gen. Microbiol 19:492
    [Google Scholar]
  8. Leith J. D.Jr 1963; Acridine orange and acriflavin inhibit deoxyribonuclease action. Biochim. biophys. Acta 72:643
    [Google Scholar]
  9. Lerman L. S. 1963; The structure of the DNA-acridine complex. Proc. nat. Acad. Sci., Wash 49:94
    [Google Scholar]
  10. Mandel H. G., Altman R. L. 1961; Some effects of chloramphenicol on biosynthesis in Bacillus cereus. J. Pharmac. exp. Therap 133:151
    [Google Scholar]
  11. Mandel H. G., Carló P. 1953; Incorporation of guanine into nucleic acids of tumorbearing mice. J. biol. Chem 201:335
    [Google Scholar]
  12. Markham R., Smith J. D. 1952; The structure of ribonucleic acids. I. Cyclic nucleotides produced by ribonuclease and by alkaline hydrolysis. Biochem. J 52:552
    [Google Scholar]
  13. Michaelson I. A., Mandel H. G. 1962; Some effects of 5-bromouracil on a thymine-requiring mutant of Escherichia coli. Biochem. Pharmacol 11:243
    [Google Scholar]
  14. Newton R. A. 1957; The mode of action of phenanthridines: the effect of ethidium bromide on cell division and nucleic acid synthesis. J. gen. Microbiol 17:718
    [Google Scholar]
  15. Ormerod W. E. 1951; A study of basophilic inclusion bodies produced by chemo-therapeutic agents in trypanosomes. Brit. J. Pharmacol 6:334
    [Google Scholar]
  16. Oyama V. I., Eagle H. 1956; Measurement of cell growth in tissue culture with a phenol reagent (Folin-Ciocalteau). Proc. Soc. exp. Biol., N.Y 91:305
    [Google Scholar]
  17. Richmond M. H. 1959; Effect of inhibitors on lytic enzyme synthesis by Bacillus subtilis. Biochim. biophys. Acta 34:325
    [Google Scholar]
  18. Roberts R. B., Abelson P. H., Cowie D. B., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in E. coli. Publ. Carneg. Instn, Wash607
    [Google Scholar]
  19. Roodyn D. B., Mandel H. G. 1960a; A simple membrane fractionation method for determining the distribution of radioactivity in chemical fractions of Bacillus cereus. Biochim. biophys. Acta 41:80
    [Google Scholar]
  20. Roodyn D. B., Mandel H. G. 1960b; The differential effect of 8-azaguanine on cell wall and protoplasmic protein synthesis in Bacillus cereus. J. biol. Chem 235:2036
    [Google Scholar]
  21. Schneider W. C. 1945; Phosphorus compounds in animal tissue. I. Extraction and estimation of desoxypentose-nucleic acid and of pentose nucleic acid. J. biol. Chem 161:293
    [Google Scholar]
  22. Seaman A., Woodbine M. 1955; The therapeutic index of chemotherapeutic agents. The effect of nucleic acid on dimidium bromide. J. appl. Bact 18:397
    [Google Scholar]
  23. Smith J. D., Matthews R. E. F. 1957; The metabolism of 8-azapurines. Biochem. J 66:323
    [Google Scholar]
  24. Tomchick R., Mandel H. G. 1962; The growth inhibition of ethidium bromide in Bacillus cereus. Proc. Am. Assoc. Cancer Res 3:368
    [Google Scholar]
  25. Tomchick R., Mandel H. G. 1963; Effects produced by ethidium bromide in Bacillus cereus and Escherichia coli. Abstr. 3rd Intersci. Conf. antimicrob. Agents Chemother p 64
    [Google Scholar]
  26. Umbreit W. W., Burris R. H., Stauffer J. F. 1957 Manometric Techniques, 3rd ed. Minneapolis: Burgess Publishing Company;
    [Google Scholar]
  27. Work E. 1951; The isolation of α,ε-di&minopimelic acid from Corynebaderiumdiphtheriae and Mycobacterium tuberculosis. Biochem. J 49:17
    [Google Scholar]
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