1887

Abstract

Summary: Qualitative and quantitative analyses of formamide extracts of group F streptococci revealed rhamnose, glucose, galactose, glucosamine and galactosamine as main components. The rhamnose content was high. Streptococci, carrying a type antigen in the cell wall, excreted into the medium also a polysaccharide with the same serological activity. These soluble polysaccharides contained no muramic acid, small amounts of rhamnose, but had a high glucosamine and mannose content. Column chromatography on DEAE-cellulose revealed the formamide extracts to be heterogeneous. Over 90 % of the serological activity could be recovered in one of the fractions. Inhibition reactions of the quantitative precipitation with a number of simple sugars gave indications about the composition of the determinant groups of group and type antigens. Rhamnose was inactive in all cases. The determinant group of the group F antigen is at least a disaccharide with a β-glucosidic moiety. The determinants of the type antigens I and II probably contain N-acetyl-galactosamine. There is evidence for a second determinant in type antigen II. The determinants of type antigens III and V contain a β-glucosidic moiety, that of antigen IV a β-galactosidic moiety.

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/content/journal/micro/10.1099/00221287-36-1-95
1964-07-01
2019-10-20
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-36-1-95
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