1887

Abstract

SUMMARY

Qualitative and quantitative analyses of formamide extracts of group F streptococci revealed rhamnose, glucose, galactose, glucosamine and galactosamine as main components. The rhamnose content was high. Streptococci, carrying a type antigen in the cell wall, excreted into the medium also a polysaccharide with the same serological activity. These soluble polysaccharides contained no muramic acid, small amounts of rhamnose, but had a high glucosamine and mannose content. Column chromatography on DEAE-cellulose revealed the formamide extracts to be heterogeneous. Over 90 % of the serological activity could be recovered in one of the fractions. Inhibition reactions of the quantitative precipitation with a number of simple sugars gave indications about the composition of the determinant groups of group and type antigens. Rhamnose was inactive in all cases. The determinant group of the group F antigen is at least a disaccharide with a -glucosidic moiety. The determinants of the type antigens I and II probably contain -acetyl-galactosamine. There is evidence for a second determinant in type antigen II. The determinants of type antigens III and V contain a -glucosidic moiety, that of antigen IV a -galactosidic moiety.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-36-1-95
1964-07-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/36/1/mic-36-1-95.html?itemId=/content/journal/micro/10.1099/00221287-36-1-95&mimeType=html&fmt=ahah

References

  1. Araujo P., Krause R. M. 1963; Group specific carbohydrate of group C-variant hemolytic streptococci. J. exp. Med 118:1059
    [Google Scholar]
  2. Cummins C. S., Harris H. 1956; The chemical composition of the cell wall in some gram-positive bacteria and its possible value as a taxonomic character. J. gen. Microbiol 14:583
    [Google Scholar]
  3. Dische Z., Sttettles L. B., Osnos M. 1949; New specific color reactions of hexoses and spectrophotometric micromethods for their determination. Archs Biochem 22:169
    [Google Scholar]
  4. Fuller A. T. 1938; The formamide method for the extraction of polysaccharides from haemolytic streptococci. Br. J. exp. Path 19:130
    [Google Scholar]
  5. Gardell S. 1953; Separation on Dowex 50 ion exchange resin of glucosamine and galactosamine and their quantitative determination. Acta chem. scand 7:207
    [Google Scholar]
  6. Gibbons M. N. 1955; The determination of methylpentoses. Analyst, Lond 80:268
    [Google Scholar]
  7. Hugett A. St G., Nixon D. A. 1957; Use of glucose oxidase, peroxidase, and O- dianisidine in determination of blood and urinary glucose. Lancet ii:368
    [Google Scholar]
  8. Krause R. M., McCarty M. 1962; Studies on the chemical structure of the streptococcal cell wall. II. The composition of group C cell walls and chemical basis for serological specificity of the carbohydrates moiety. J exp. Med 115:49
    [Google Scholar]
  9. Kündig W., Neukom H., Deuel H. 1961; Untersuchungen über Getreideschleim- stoffe. I. Chromatographische Fraktionierung von wasserlöslichen Weizenmehl- pentosanen an Diäthylaminoäthyl-Cellulose. Helv. chim. Acta 44:823
    [Google Scholar]
  10. McCarty M., Lancefield R. 1955; Variation in the group-specific carbohydrate of Group A streptococci. I. Immunochemical studies on the carbohydrates of variant strains. J. exp. Med 102:11
    [Google Scholar]
  11. McCarty M. 1956; Variation in the group-specific carbohydrate of group A streptococci. II. Studies on the chemical basis for serological specificity of carbohydrates. J. exp. Med 104:629
    [Google Scholar]
  12. Michel M. F., Gooder H. 1962; Amino acids, amino sugars and sugars present in the cell wall of some strains of Streptococcus pyogenes. J. gen. Microbiol 29:199
    [Google Scholar]
  13. Ottens H. 1961 Indifferente en haemolytische streptococcen met groepsantigeen F Thesis University of Utrecht:
    [Google Scholar]
  14. Ottens H., Winkler K. C. 1962; Indifferent and haemolytic streptococci possessing group antigen F. J. gen. Microbiol 28:181
    [Google Scholar]
  15. Rondle C. I. M., Morgan W. T. I. 1955; The determination of glucosamine and galactosamine. Biochem. J 61:586
    [Google Scholar]
  16. Slade H. D., Slamp W. C. 1962; Cell-wall composition and the grouping antigens of streptococci. J. Bact 84:345
    [Google Scholar]
  17. Trevelyan W. E., Procter D. P., Harrison I. S. 1950; Detection of sugars on paper chromatograms. Nature; Lond: 166444
    [Google Scholar]
  18. Winkler K. C., Van Amerongen J. 1959; Bacteriological results from 4000 root canal cultures. Oral Surg 12:857
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-36-1-95
Loading
/content/journal/micro/10.1099/00221287-36-1-95
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error