1887

Abstract

SUMMARY: The effect of cultural conditions on the growth and nitrate reductase activity of a bacterium, now identified as , were examined. The bacterium grew well with ammonium chloride, ammonium nitrate or potassium nitrate as sole nitrogen source. Nitrate reductase activity was markedly decreased in organisms deficient in molybdenum or iron and in organisms grown at high oxygen pressures. The enzyme is thus similar to nitrate reductases from other bacteria. The enzyme is constitutive; it is present in organisms grown with ammonium chloride in the absence of nitrate; the ammonium radical did not depress enzyme activity. Under certain conditions (NHNO medium, 10%, v/v, O in N) molybdenum (10 g./l.) depressed the nitrate reductase activity, but this effect was annulled by repeated subcultivation in molybdenum-deficient medium. Vanadium and especially tungsten, also inhibited the enzyme. The uptake of nitric oxide was decreased in organisms depleted of iron but was increased in organisms deficient in molybdenum. The behaviour of nitrate reductase preparations during fractionation whether from grown with KNO or with NHCl suggests that the enzyme is the same from both sources.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-35-3-401
1964-06-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/35/3/mic-35-3-401.html?itemId=/content/journal/micro/10.1099/00221287-35-3-401&mimeType=html&fmt=ahah

References

  1. Bergey’s Manual of Determinative Bacteriology 1957 7th ed. Ed. by R. S. Breed, E. G. D. Murray & N. R. Smith. Baltimore: Williams and Wilkins Co.
  2. Fewson C. A., Nicholas D. J. D. 1960; Utilisation of nitric oxide by micro-organisms and higher plants.. Nature, Lond. 188:794
    [Google Scholar]
  3. Fewson C. A., Nicholas D. J. D. 1961; Nitrate reductase from Pseudomonas aeruginosa.. Biochim. biophys. Acta 49:335
    [Google Scholar]
  4. Higgins E. S., Richert D. A., Westerfeld W. W. 1956; Tungstate antagonism of molybdate in Aspergillus niger.. Proc. Soc. exp. Biol., N.Y. 92:509
    [Google Scholar]
  5. Kinsky S. C. 1961; Induction and repression of nitrate reductase in Neurospora crassa.. J. Bact. 82:989
    [Google Scholar]
  6. Lindeberg G., Lode A., Somme R. 1963; Effect of oxygen on formation and activity of nitrate reductase in a halophilic Achromobacter species.. Acta chem. scand. 17:232
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent.. J. Mol. Chem. 193:265
    [Google Scholar]
  8. Medina A., Nicholas D. J. D. 1957; Interference by reduced pyridine nucleotides in the diazotization of nitrite.. Biochim. biophys. Acta 23:440
    [Google Scholar]
  9. Nicholas D. J. D. 1952; The use of fungi for determining trace metals in biological materials.. Analyst, Lond. 77:629
    [Google Scholar]
  10. Nicholas D. J. D., Medina A. 1960; A nitrate reductase from Neurospora crassa.. Biochim. biophys. Acta 37:468
    [Google Scholar]
  11. Nicholas D. J. D., Nason A. 1954; Mechanism of action of nitrate reductase from Neurospora.. J. biol. Chem. 211:183
    [Google Scholar]
  12. Nicholas D. J. D. 1961; Minor mineral nutrients.. A. Rev. PI. Physiol. 12:63
    [Google Scholar]
  13. Nicholas D. J. D. 1963; The metabolism of inorganic nitrogen and its compounds in micro-organisms.. Biol. Rev. 38:530
    [Google Scholar]
  14. Nicholas D. J. D., Redmond W. J., Wright M. A. 1963; Molybdenum and iron requirements for nitrate reductase in Photobacterium sepia.. Nature, Lond. 200:1125
    [Google Scholar]
  15. Pichinoty F., d’Ornano L. 1961a; Influence des conditions de culture sur la formation dela nitrate reductase d’Aerobacter aerogenes.. Biochim. biophys. Acta 48:218
    [Google Scholar]
  16. Pichinoty F., d’Ornano L. 1961b; Inhibition by oxygen of biosynthesis and activity of nitrate reductase in Aerobacter aerogenes.. Nature, Lond. 191:879
    [Google Scholar]
  17. Sadana J. C., McElroy W. D. 1957; Nitrate reductase from Achromobacter fischeri. Purification and properties: Function of flavines and cytochrome.. Archs. Biochem. 67:16
    [Google Scholar]
  18. San Pietro A. 1957; The measurement of stable isotopes.. Meth. Enzymol. 4:473
    [Google Scholar]
  19. Sato R. 1956 In Inorganic Nitrogen Metabolism Ed. by W. D. McElroy & B. Glass 163 The Johns Hopkins Press;
    [Google Scholar]
  20. Simms P., Cocking C. 1958; Assay of isotopic nitrogen by mass-spectrometer.. Nature, Lond. 181:474
    [Google Scholar]
  21. Spencer R. 1955; Taxonomy of certain luminous bacteria.. J. gen. Microbiol. 13:111
    [Google Scholar]
  22. Spencer R. A. 1935; Nutrient solution purification for removal of heavy metals in deficiency investigations with Aspergillus niger.. J. agric. Res. 51:413
    [Google Scholar]
  23. Takahashi H., Nason A. 1957; Tungstate as a competitive inhibitor of molybdate in nitrate assimilation and in N2 fixation by Azotobacter.. Biochim. biophys. Acta 23:433
    [Google Scholar]
  24. Verhoeven W. 1952; Aerobic sporeforming nitrate reducing bacteria. Thesis. University of Delft.. Biochim. biophys. Acta 23:433
    [Google Scholar]
  25. Walker G. C., Nicholas D. J. D. 1961; An iron requirement for a dissimilatory nitrate reductase in Neurospora crassa.. Nature, Lond. 189:141
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-35-3-401
Loading
/content/journal/micro/10.1099/00221287-35-3-401
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error