1887

Abstract

SUMMARY: An electron microscopic investigation was carried out on thin sections of both S and R variants of , strain : no ultrastructural differences were noted between the two variants.

The cells are limited by a cell wall, comprising an outer triple-layered membrane (65–80 Å. thick) and an inner homogeneous layer of variable thickness, and a triple-layered cytoplasmic membrane about 70 Å. thick. The cytoplasm and nucleoplasm of brucellae are similar to those of other bacteria. A characteristic feature of these organisms is the presence of membranous structures (300–800 Å. in diameter) associated with the cytoplasmic membrane. These “peripheral formations”, which occur singly or in clusters, do not appear to arise by obvious invagination of the cytoplasmic membrane.

No evidence has been obtained for the existence of capsules in virulent brucellae.

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1964-06-01
2022-01-27
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References

  1. Borries B. von, Ruska E., Ruska H. 1938; Bakterien und Virus in übermikro-scopischer Aufnahme.. Klin. Wschr. 17:921
    [Google Scholar]
  2. Braun W., Pomales-Lébron A., Stinebring W. R. 1958; Interaction between mononuclear phagocytes and Brucella abortus strains of different virulence.. Proc. Soc. exp. Biol., N.Y. 97:393
    [Google Scholar]
  3. Brown A. D., Drummond D. G., North R. J. 1962; The peripheral structure of Gramnegative bacteria. II. Membranes of bacilli and spheroplasts of a marine pseudomonad.. Biochim. biophys. Acta 58:514
    [Google Scholar]
  4. Caro L. G. 1961; Localization of macromolecules in Escherichia coli. I. DNA and proteins.. J. biophys. biochem. Cytol. 9:539
    [Google Scholar]
  5. Chapman G. B., Kroll A. J. 1957; Electron microscopy of ultrathin sections of Spirillum serpens.. J. Bad. 73:63
    [Google Scholar]
  6. Clarke P. H., Lilly M. D. 1962; A general structure for cell walls of Gram-negative bacteria.. Nature, Lond. 195:516
    [Google Scholar]
  7. Conti S. F., Gettner M. E. 1961; Electron microscopy of cellular division in Escherichia coli.. J. Bad. 83:544
    [Google Scholar]
  8. Fitz-James P. 1960; Participation of the cytoplasmic membrane in the growth and spore formation of bacilli.. J. biophys. biochem. Cytol. 8:507
    [Google Scholar]
  9. Glauert A. M., Glauert R. H. 1958; Araldite as an embedding medium for electron microscopy.. J. biophys. biochem. Cytol. 4:191
    [Google Scholar]
  10. Glauert A. M., Brieger E. M., Allen J. M. 1961; The fine structure of vegetative cells of B. subtilis. Exp.. Cell Res. 22:73
    [Google Scholar]
  11. Glauert A. M. 1962; The fine structure of bacteria.. Brit. med. Bull. 18:245
    [Google Scholar]
  12. Huddleson I. F. 1940; The presence of a capsule on brucella cells.. J. amer. vet. med. Assoc. 96:708
    [Google Scholar]
  13. Huddleson I. F. 1941; The presence of a capsule on brucella cells.. Tech. Bull. Mich, agrie. Exp. Sta. 177:11
    [Google Scholar]
  14. Huxley H. E., Zubay G. 1960; Electron microscope observations on the structure of microsomal particles from Escherichia coli.. J. molec. Biol. 2:10
    [Google Scholar]
  15. Karlsbad G., Kessel R. W. I., DE Petriss S., Monaco L. 1964; Electron microscope observations of Brucella abortus grown within monocytes in vitro.. J. gen. Microbiol. 35:
    [Google Scholar]
  16. Kellenberger E., Ryter A. 1958; Cell wall and cytoplasmic membrane of Escherichia coli.. J. biophys. biochem. Cytol. 4:323
    [Google Scholar]
  17. Kellenberger E., Ryter A., Séchaud J. 1958; Electron microscopic study of DNA-containing plasms. II. Vegetative and mature phage DNA as compared with normal bacterial nucleoids in different physiological states.. J. biophys. biochem. Cytol. 4:971
    [Google Scholar]
  18. Lembke A., Kornlein M., Frahn H. 1950; Beitrag zum Brucellose-Problem.. Z. Baht. 155:16
    [Google Scholar]
  19. Luft J. H. 1956; Permanganate—a new fixative for electron microscopy.. J. biophys. biochem. Cytol. 2:799
    [Google Scholar]
  20. Malfatti M. G., Ithurralde D. 1951; Valor taxonómica de las imágenes electrónicas de las bacterias de género Brucella.. Sem. méd., B. Aires 98:704
    [Google Scholar]
  21. Mickle W. A. 1940; Capsule formation by members of the brucella group.. J. infect. Dis. 66:271
    [Google Scholar]
  22. Robertson J. D. 1959; The ultrastructure of cell membranes and their derivatives.. Biochem. Soc. Symp. 16:3
    [Google Scholar]
  23. Ryter A., Kellenberger E., Birsch-Andersen A., Maaloe O. 1958; Étude au microscope électronique de plasmas contenant de l’acide désoxyribonucléique. I. Les nucléoides des bactéries en croissance active.. Z. Naturforsch. 13B:597
    [Google Scholar]
  24. Saltón M. R. J. 1962; Cell wall structure and biosynthesis.. J. gen. Microbiol. 29:15
    [Google Scholar]
  25. Scanga F. 1958; The relationship of antigenic structure to anatomy in Salmonellae.. Abstr. 7th int. Congr. Microbiol. p. 430
    [Google Scholar]
  26. Shinohara C., Fukushi K., Suzuki J. 1957; Mitochondria-like structures in ultrathin sections of Mycobacterium avium.. J. Bad. 74:413
    [Google Scholar]
  27. Stinebring W. R., Braun W. 1959; Brucellaphage.. J. Bad. 78:736
    [Google Scholar]
  28. Suganuma A. 1961; The plasma membrane of Staphylococcus aureus.. J. biophys. biochem. Cytol. 10:292
    [Google Scholar]
  29. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of E. coli strain B.. J. gen. Microbiol. 22:158
    [Google Scholar]
  30. Wyss O., Neumann M. G., Socolofski M. D. 1961; Development and germination of the Azotobader cyst.. J. biophys. biochem. Cytol. 10:555
    [Google Scholar]
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