Summary: Cells of Gram-negative bacteria undergo lysis when treated with lysozyme in the presence of ethylenediaminetetraacetic acid (EDTA) and tris buffer, as shown by Repaske. However, contrary to the prevalent assumption, lysis is not necessarily preceded by formation of a spheroplast as the cell wall is damaged. Treatment of Escherichia coli and Pseudomonas aeruginosa with the lytic system was shown to cause the formation of osmotically fragile rods, rather than spheres. The extent of destruction of the cell walls of Gram-negative bacteria by lysozyme in this system is, at least in some cases, less than has been generally supposed.
FraserD.,
MahlerH. R.1957; The effects of nucleases on the reproduction of T3 bacteriophage in protoplasts of Escherichia coli
. Archs. Biochem. Biophys. 69:166
McQuillenK.1960; Bacterial protoplasts; in The Bacteria A Treatise on Structure and Function. Ed. by I. C. Gunsalus & R. Y. Stanier, vol. 1,. New York and London: Academic Press. 1:268
TulasneR.,
MinckR.,
KirnA.,
KrembelJ.1960; Délimitation de la notion de formes L des bactéries: protoplastes, sphéroplastes et formes L. Ann.. Inst. Pasteur, 99:859