1887

Abstract

Summary: organisms, grown in basal medium of water activity (a) 0·993 and in basal medium adjusted to several different a values by addition of NaCl, were analysed for the following components: sodium, potassium, calcium, magnesium, total amino acids, inorganic phosphate, chloride, water, DNA, RNA, protein. Cell water decreased from 1·66 to 0·83 g./g. dry weight when a was decreased from 0·993 to 0·90. Internal concentrations of solutes generally increased with decrease in a, potassium and phosphate concentrations being greatest at 0·92 a and sodium, chloride, magnesium, and amino acids at 0·90 a, the lowest a value studied. Increases in potassium and amino acid concentrations resulted largely from the decreased water contents of cocci grown at low a values. Intracellular sodium and chloride concentrations were much lower than and proportional to the NaCl concentration in the medium. The predominant cell solute was potassium at 0·92 a and above, and sodium below 0·92 a. The data are discussed in relation to the inhibition of bacterial growth at low a values.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-35-2-205
1964-05-01
2019-12-06
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-35-2-205
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error