Summary: A method is described whereby pH and E of a medium can be closely controlled during the growth of type A for long enough to study the bacteriostatic effects of horse antiserum. The pH value was controlled manually to about ± 0·01 unit (S.D.), by adjustments in the CO tension of the gas above the medium. The E was controlled by the addition of oxygen to a system with an inherent tendency to become reduced. With manual control the E was maintained within ± 2 mV (S.D.); automatic control gave better regulation than this.


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