1887

Abstract

Summary: A method is described whereby pH and E of a medium can be closely controlled during the growth of type A for long enough to study the bacteriostatic effects of horse antiserum. The pH value was controlled manually to about ± 0·01 unit (), by adjustments in the CO tension of the gas above the medium. The E was controlled by the addition of oxygen to a system with an inherent tendency to become reduced. With manual control the E was maintained within ± 2 mV (); automatic control gave better regulation than this.

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/content/journal/micro/10.1099/00221287-35-2-169
1964-05-01
2022-01-17
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References

  1. Bullen J. J., Dobson A. 1962; Bacteriostatic properties of sera and immunity to Clostridium welchii type a.. Colston Pap. 13:123
    [Google Scholar]
  2. Bullen J. J., Dobson A. 1963; The role of Eh and pH in bacteriostatic effects of horse antiserum on Clostridium welchii type a.. Biochem. J. 88:11
    [Google Scholar]
  3. Bullen J. J., Dobson A., Wilson A. B. 1964; Bacteriostatic effects of horse antiserum on Clostridium welchii type a. The role of Eh and pH.. J. gen. Microbiol. 35:175
    [Google Scholar]
  4. Hanke M. E., Katz Y. J. 1943; An electrolytic method for controlling oxidation-reduction potential and its application to the study of anaerobiosis.. Arch. Biochem. 2:183
    [Google Scholar]
  5. Hanke M. E., Bailey J. H. 1945; Oxydation-reduction potential requirements of Cl. welchii and other Clostridia.. Proc. Soc. exp. Biol., N.Y. 59:163
    [Google Scholar]
  6. Knight B. C. J. G. 1930; Oxidation-reduction studies in relation to bacterial growth. II. A method of poising the oxidation-reduction potential of bacteriological culture media.. Biochem. J. 24:1075
    [Google Scholar]
  7. Knight B. C. J. G. 1931; Oxidation-reduction potential measurement in cultures and culture media. In A System of Bacteriology 9:165 Ed. by P. Fildes & J. McIntosh. London: H.M. Stationery Office.
    [Google Scholar]
  8. Knight B. C. J. G., Fildes P. 1930; Oxidation-reduction studies in relation to bacterial growth. III. The positive limit of oxidation-reduction potential required for the germination of B. tetani spores in vitro.. Biochem. J. 24:1496
    [Google Scholar]
  9. Vennesland B., Hanke M. E. 1940; The oxidation-reduction potential requirements of a non-spore-forming, obligate anaerobe.. J. Bact. 39:139
    [Google Scholar]
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