Washed suspensions of incubated anaerobically in the presence of penicillin and neomycin incorporated C-glycine in the cell ‘pool’ as -acetylglycine and into the cell protein as glycine without conversion to any other amino acid. At low external concentrations the glycine was present in the protozoa themselves and was not free in the gastric sac or present solely in the intracellular or extracellular bacteria. The incorporation reached a constant value after incubation for 24 hr; after this time the uptake of glycine was balanced by an equal loss of -acetylglycine from the organisms. These suspensions also incorporated C from NaHCO into carbon atoms 3 and 4 of protozoal glucose which was probably present as starch in the intact protozoa.


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