1887

Abstract

Washed suspensions of incubated anaerobically in the presence of penicillin and neomycin incorporated C-glycine in the cell ‘pool’ as -acetylglycine and into the cell protein as glycine without conversion to any other amino acid. At low external concentrations the glycine was present in the protozoa themselves and was not free in the gastric sac or present solely in the intracellular or extracellular bacteria. The incorporation reached a constant value after incubation for 24 hr; after this time the uptake of glycine was balanced by an equal loss of -acetylglycine from the organisms. These suspensions also incorporated C from NaHCO into carbon atoms 3 and 4 of protozoal glucose which was probably present as starch in the intact protozoa.

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1964-04-01
2022-01-17
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References

  1. Abou Akkada A.R, Howard B.H. 1962; The biochemistry of rumen protozoa. 5. The nitrogen metabolism of Entodinium.. Biochem. J. 82:313
    [Google Scholar]
  2. Abrahama S., Hassid W.Z. 1957; The synthesis and degradation of isotopically labelled carbohydrates and carbohydrate intermediates. Methods in Enzymology Ed. by S. P. Colowick & N. O. Kaplan 4 New york: Academic Press Inc;
    [Google Scholar]
  3. Alexander B., Landwehr G., Seligman A.M. 1945; A specific micromethod for the colorimetric determination of glycine in blood and urine.. J. biol. Chem. 160:51
    [Google Scholar]
  4. Bach J.S. 1952 The Metabolism of Protein Constituents in the Mammalian Body Oxford: Clarendon Press;
    [Google Scholar]
  5. Bandurski R.S., Axelrod B. 1951; The chromatographic identification of some biologically important phosphate esters.. J. biol. Chem. 193:405
    [Google Scholar]
  6. Barnet H.N., Wick A.N. 1950; The formation of glycogen from 14C-labelledg lycine.. J. biol. Chew. 185:657
    [Google Scholar]
  7. Coleman G.S. 1956; The dissimilation of amino acids by Rhodospirillum rubrum. . J. gen. Microbiol. 15:248
    [Google Scholar]
  8. Coleman G.S. 1958; Maintenance of oligotrich protozoa from the sheep rumen in vitro. . Nature, Lond. 182:1104
    [Google Scholar]
  9. Coleman G.S. 1960a; The cultivation of sheep rumen oligotrich protozoa in vitro. . J. gen. Microbiol. 22:555
    [Google Scholar]
  10. Coleman G.S. 1960b; Effect of penicillin on the maintenance of rumen oligotrich protozoa.. Nature, Lond. 187:518
    [Google Scholar]
  11. Coleman G.S. 1962; The preparation and survival of bacteria-free suspensions of Entodinium caudatum. . J. gen. Microbiol 28:271
    [Google Scholar]
  12. Dewey V.C., Kidder G.W. 1955; Threonine aldolase in Tetrahymena. Résumés communs. 3e Congr. int. Biochem. Brussels92
    [Google Scholar]
  13. Elsden S.R., Lewis D. 1953; The production of fatty acids by a Gram-negative coccus.. Biochem. J. 55:183
    [Google Scholar]
  14. Guillaume J., Osteux R. 1955; Détermination des acides aliphatiques volatils de C1 á C6 dans les liquids biologiques par chromatographie sup papier.. C.R. Acad. Sci., Paris, 241:501
    [Google Scholar]
  15. Gutierrez J., Davis R.E. 1962; Culture and metabolism of the rumen ciliate Epidinium ecaudatum (Crawley). Appl. Microbiol 10:305
    [Google Scholar]
  16. Hardy T.L, Holland D.O, Nayler J.H.C. 1955; One-phase solvent mixtures for separation of amino acids.. Analyt. Chem 27:971
    [Google Scholar]
  17. Horrocks R.H, Manning G.B. 1949; Partition chromatography on paper. Identification of reducing substances in urine.. Lancet i:1042
    [Google Scholar]
  18. Huggett A.St.G., Nixon D.A. 1957; Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose.. Lancet,ii–368
    [Google Scholar]
  19. Park J.T. 1958; Selective inhibition of bacterial cell wall synthesis: its possible application in chemotherapy. In Strategy of Chemotherapy. . Symp. Soc.gen. Microbiol. 8:49
    [Google Scholar]
  20. Potter V.R., Elvehjem C.A. 1936; A modified method for the study of tissue oxidations.. J. biol. Chern. 114:495
    [Google Scholar]
  21. Roberts R.B., Abelson P.H., Cowie D.B., Bolton E.T., Britten R.J. 1955; Studies on Biosynthesis in Escherichia coli.. Publ. Carneg. Inst. no.607
    [Google Scholar]
  22. Schneider W.C. 1945; Phosphorous compounds in animal tissue. 1. Extraction and estimation of desoxypentose nucleic acid and of pentose nucleic acid.. J. bioZ. Chem. 161:293
    [Google Scholar]
  23. Williams P.P., Davis R.E., Doetsch R.N., Gutierrez J. 1961; Physiological studies of the rumen protozoon Ophyroscolex caudatus (Eberlein).. Appl. Microbiol. 9:405
    [Google Scholar]
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