1887

Abstract

Ascorbic acid undergoing auto-oxidation inactivated vaccinia virus. Copper ion was shown to have a catalytic effect on the inactivation. Neither unoxidized ascorbic acid nor its oxidation product, dehydroascorbic acid, were inhibitory. When ascorbic acid was oxidized at high pH in the absence of copper ion no inactivation took place. Similarly, enzymic oxidation of ascorbic acid in the absence of copper was without effect on the virus. Catalase prevented inactivation but not the oxidation of ascorbic acid. Glutathione prevented both inactivation and the oxidation of ascorbic acid. Inhibition experiments with ascorbic acid under anaerobic conditions were inconclusive. The mechanism of ascorbic acid inactivation is discussed in the light of these data and that of other authors with different viruses.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-35-1-75
1964-04-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/35/1/mic-35-1-75.html?itemId=/content/journal/micro/10.1099/00221287-35-1-75&mimeType=html&fmt=ahah

References

  1. Barron E.S.G., Demilio R.H., Klemperer F. 1936; Studies on biological oxidations. V. Copper and haemochromogens as catalysts for the oxidation of ascorbic acid. The mechanism of the oxidation.. J. biol. Chem. 113:625
    [Google Scholar]
  2. Berneis K. 1963; Der Abbau von Desoxyribo-nucleinsäure durch Ascorbinsäure: Die intermediäre Bildung von OH-Radikalen.. Helv. chim. Actu, 46:57
    [Google Scholar]
  3. Holden M., Resnick R. 1936; The in vitro action of synthetic crystalline vitamin C (ascorbic acid) on Herpes virus.. J. Immunol. 31:455
    [Google Scholar]
  4. Holden M., Molloy E. 1937; Further experiments on the inactivation of Herpes virus by vitamin C (ascorbic acid).. J. Immunol. 33:251
    [Google Scholar]
  5. Jungeblut C.W. 1935; Inactivation of poliomyelitis virus in vitro by crystalline vitamin C (ascorbic acid).. J. exp. Med. 62:517
    [Google Scholar]
  6. Kligler I.J, Bernkopf H. 1937; Inactivation of vaccinia virus by ascorbic acid and glutathione.. Nuture, Lond. 139:965
    [Google Scholar]
  7. Lojkin M. 1936; A study of ascorbic acid as an inactivating agent of tobacco mosaic virus.. Contr. Boyce Thompson Inst. Pl. Res. 8:455
    [Google Scholar]
  8. Lominski I. 1936; Inactivation du bacteriophage par l’acide ascorbique.. C. r. Seanc. Soc. Biol. 122:766
    [Google Scholar]
  9. Milas N.A. 1932; Auto-oxidation.. Chem. Rev. 10:295
    [Google Scholar]
  10. Peloux Y., Lofre C., Cier A., Colobert A. 1962; Inactivation du virus poliomyélitique par des systémes chimique générateurs du radical libre hydroxyle. Méchanism de l’activité virulicide du peroxyde d’hydrogéne et de l’acide ascorbique.. Annls Inst. Pasteur, Paris,. 102:6
    [Google Scholar]
  11. Reed L.J., Muench H. 1938; A simple method for estimating fifty per cent end points.. Am. J. Hyg. 27:493
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-35-1-75
Loading
/content/journal/micro/10.1099/00221287-35-1-75
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error