Repression by Methionine of Cystathionase Formation in Free

Abstract

Cystathionase catalyses the formation of homocysteine from cystathionine; its formation in cultures of is repressed by the presence of methionine in the growth medium, and to a similar extent to that shown with homocysteine methylase (the enzyme complex which catalyses the conversion homocysteine → methionine). Cystathionase, again like homocysteine methylase, is formed rapidly without concomitant growth when repressed organisms are transferred to a medium free from methionine; such enzyme formation is prevented by chloramphenicol, suggesting that synthesis of protein is required. The co-repression by methionine of the two enzyme systems fortifies the evidence that cystathionase is a component of the normal pathway of methionine synthesis by and consequently that its substrate, cystathionine, is a normal intermediate. Cystathionase preparations also formed pyruvate from cysteine; this activity paralleled that of cystathionase itself when the methionine status of the medium was changed, and it is concluded that the same protein is responsible for both activities.

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1964-04-01
2024-03-29
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