A complex tissue culture medium supplemented with swine serum and peptone supported optimal growth of strain 293. Media lacking any of these components supported little or no growth. However, when 5′-monophosphate nucleotides replaced the peptone growth was supported. The minimal nucleotides necessary to support good growth were adenylic, cytidylic, guanylic and thymidylic acids. In some cases the addition of the four ribonucleotides and the four deoxyribonucleotides in place of peptone improved growth; the four ribonucleotides alone supported poor growth. Thymidylic acid seemed essential for growth, and uridylic acid appeared to be inhibitory.

The mixture of ribonucleosides and deoxyribonucleosides, but not of purines and pyrimidines, when substituted for the peptone, also supported good growth. The concentrations of nucleosides and nucleotides had a significant effect on growth. Although the nutritional factors of swine serum were not defined, the effects of different sera on growth were investigated. Rabbit, horse, turkey and swine serum supported optimum growth; human serum supported less, whereas PPLO serum fraction (Difco) or bovine serum supported poor growth. Dog serum did not support growth.


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