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Volume 35, Issue 1

Further Studies of Ultraviolet-sensitive Mutants of Strain B Free

Abstract

A group of twelve ultraviolet-sensitive mutants has been isolated after u.v. irradiation of strain b. The use of crystal violet during irradiation and subsequent growth appears to increase the frequency of such mutants among colonies formed by surviving cells. This effect may be due to post-irradiation selection. Eleven of the mutants were more crystal violet resistant than their parent.

The mutants were compared by determining their u.v. survival curves, the extent of elongation after u.v. irradiation, their ability to repair u.v. induced damage to bacteriophage T1, and their resistance to furacin. They comprised nine different phenotypes. In all but one case, the mutants differed from the parent in more than one property.

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© Society for General Microbiology, 1964
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1964-04-01
2025-07-08
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References

  1. Adams M.H. 1950; Methods of study of bacterial viruses.. Meth. med. Res. 2:1
     [Google Scholar]
  2. Adler H.I, Copeland J.C. 1962; Genetic analysis of radiation response in Escherichia coli.. Genetics 47:701
     [Google Scholar]
  3. Bryson V, Rosenblum E, Kaplan S., Hershey H., Cuneo H., Dittman I. 1951-52; Genetic and biochemical studies of bacteria. Long Island Biol. Assoc. Annu. Rep. Biol. Lab.24
     [Google Scholar]
  4. Campbell A. 1961; Sensitive mutants of bacteriophage lambda.. Virology 14:22
     [Google Scholar]
  5. Curry J., Greenberg J. 1962; Filament formation in radioresistant mutants of Escherichia coli s after treatment with ultraviolet light and radiomimetic agents.. J. Bact. 83:38
     [Google Scholar]
  6. Demerec M. 1956; A comparative study of certain gene loci in Salmonella.. Cold Spr. Harb. Symp. quant. Biol. 21:113
     [Google Scholar]
  7. Garen A., Zinder N.D. 1955; Radiological evidence for partial genetic homology between bacteriophage and host bacteria.. Virology 1:347
     [Google Scholar]
  8. Goebel W.F, Jesaitis M.A. 1952; Chemical and antiviral properties of the somatic antigen of phase I1 ‘Sh. sonnei’ and of a phage-resistant variant, II/3,4,7. Le Bacteriophage.. Premier Colloque International at Royaumont66
     [Google Scholar]
  9. Greenberg J., Woody-Karrer P. 1963; Radioresistant mutants of Escherichia coli B (ORNL).. Radiat. Res. 20:350
     [Google Scholar]
  10. Harm W. 1963; On the relationship between host-cell reactivation and UV-reactivation in UV-inactivated phages.. Z. Vererb 94:67
     [Google Scholar]
  11. Hili R.F., Rossi H.H. 1952; Absence of photoreactivation in Tl bacteriophage irradiated with ultraviolet in the dry state.. Science, 116:424
     [Google Scholar]
  12. Hili R.F., Simsin E. 1961; A study of radiosensitive and radio-resistant mutants of Escherichia coli strain B.. J. gen. Microbial. 24:1
     [Google Scholar]
  13. Howard-Flanders P., Theriot L. 1962; A method for selecting radiation-sensitive mutants of Escherichia coli.. Genetics 47:1219
     [Google Scholar]
  14. Howard-Flanders P., Boyce R.P., Simson E., Theriot L. 1962; A genetic locus in E. coli K 12 that controls the reactivation of UV-photoproducts associated with thymine in DNA.. Proc. nat. Acad. Sci., Wash 48:2109
     [Google Scholar]
  15. Jagger J. 1961; A small and inexpensive ultraviolet dose-rate meter useful in biological experiments.. Radiat. Res. 14:394
     [Google Scholar]
  16. Payne J.I., Hartman P.E., Mudd S., Phillips A.W. 1956; Cytological analysis of ultraviolet-irradiated Escherichia coli. III. Reactions of a sensitive strain and its resistant mutants.. J. Bact. 72:461
     [Google Scholar]
  17. Rörsch A., Edelman A., Cohen J.A. 1963; The gene-controlled radiation sensitivity in Escherichia coli.. Biochim. biophys. acta, 68:263
     [Google Scholar]
  18. Rörsch A., Edelman A., Van der kamp C., Cohen J.A. 1962; Phenotypic and genotypic characterization of radiation sensitivity in Escherichia coli B.. Biochim. biophys. acta, 61:278
     [Google Scholar]
  19. Sauerbier W. 1961; The influence of 5-bromodeoxyuridine substitution on UVsensitivity, host-cell reactivation, and photoreactivation in T1 and P22H5.. Virology, 15:465
     [Google Scholar]
  20. Szybalski W. 1962; Microbial selection. I. Gradient plate technique for study of bacterial resistance.. Science, 116:46
     [Google Scholar]
  21. Szybalski W., Nelson T.C. 1954; Genetics of bacterial resistance to nitrofurans and radiation.. Bact. proc 1954:51
     [Google Scholar]
  22. Witkin E.M. 1947; Genetics of resistance to radiation in Escherichia coli.. Genetics 32:221
     [Google Scholar]
  23. Witkin E.M. 1961; Modification of mutagenesis initiated by ultraviolet light through posttreatment of bacteria with basic dyes.. J. Cell. comp. Physiol. 58:135
     [Google Scholar]
  24. Woody-Karrer P.W., Greenberg J. 1963; Resistance and cross-resistance of Escherichia coli s mutants to the radiomimetic agent nitrofurazone.. J. Bact. 85:1208
     [Google Scholar]
  25. Yanofsky C., Lennox E.S. 1959; Transduction and recombination study of linkage relationships among the genes controlling tryptophan synthesis in Escherichia coli.. Virology 8:425
     [Google Scholar]
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Further Studies of Ultraviolet-sensitive Mutants of Escherichia coli Strain B
Microbiology 35, 105 (1964); https://doi.org/10.1099/00221287-35-1-105
/content/journal/micro/10.1099/00221287-35-1-105
/content/journal/micro/10.1099/00221287-35-1-105
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