Summary: was allowed to grow on unlabelled medium until gibberellic acid (GA) was being produced, and samples of mycelium were then transferred to a substrate containing C-acetate. Pure C-GA was isolated from the medium. The specific activity of the C-GA was increased by transferring mycelium to a second labelled substrate 6 hr after the first transfer. The specific activity was not increased when unlabelled glucose was present in the transfer medium, or when anaerobic conditions were maintained after transfer. Most of the C-GA was produced during the 48 hr following transfer. A large proportion of the C was lost as CO, and most of this was produced during the 40 hr after transfer. After this period little GA was produced, but the evolution of unlabelled CO continued.


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