SUMMARY: Homogenates of brains from mice clinically affected with scrapie have been fractionated by differential centrifugation and equilibration in sucrose density gradients. Most of the infectivity was found in the heavy particulate fraction containing mitochondria and possibly lysosomes.
GrayE. G.,
WhittakerV. P.1962; The isolation of nerve endings from the brain: an electron-microscopic study of cell fragments derived by homogenisation and centrifugation. J. Anat 96:79
HunterG. D.,
MillsonG. C.1963; The comparative infectivities of extracts of tissues and of purified cell fractions from mice infected with the scrapie agent. Biochem. J 89:42
HunterG. D.,
MillsonG. C.,
ChandlerR. L.1963; Observations on the comparative infectivity of cellular fractions derived from homogenates of mouse-scrapie brain. Res. vet. Sci (in the Press)
SchneiderH. A.1959; Genetic and nutritional aspects of experimental ‘allergic’ encephalomyelitis, in ‘Allergic’ Encephalomyelitis
. 3130. M. W. Kies and E. C. Alvord; Oxford: Blackwell Scientific Publications, Ltd:
ZlotnikI.,
RennieJ. C.1963; Further observations on the experimental transmission of scrapie from sheep and goats to laboratory mice. J. comp. Path 73:150