1887

Abstract

SUMMARY: A survey has been made of the immunological relationships between glutamate dehydrogenase (GDH) and the related proteins of the amination deficient () mutants of . Serum against GDH was prepared in rabbits which had been injected with crude extracts of wild type . The effect of this serum on the activity of normal GDH and the activity of activated mutant proteins was studied. The techniques of double diffusion in agar gel and of immunoelectrophoresis were used to study the relationships between the wild-type GDH and mutant forms of the protein. The identification of the relevant precipitation line on these gel plates was confirmed by the use of a specific stain for the enzyme antibody complex. The relationship between the antigenic and enzymic sites in the wild-type protein is discussed.

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/content/journal/micro/10.1099/00221287-34-2-295
1964-02-01
2021-10-27
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References

  1. Barratt R. W. 1962; Altered proteins produced by mutation at the amination (am) locus in Neurospora. Genetics 47:941
    [Google Scholar]
  2. Burk R. R., Pateman J. A. 1962; Glutamic and alanine dehydrogenase determined by one gene in Neurospora crassa . Nature, Lond 196:450
    [Google Scholar]
  3. Coombs R. R. A., Richards C. B., Dodd B. 1963; Serological identification of seminal stains. Medicine, Science and the Law 3:65
    [Google Scholar]
  4. Fincham J. R. S. 1959; The role of chromosomal loci in enzyme formation. Proc. X int. Congr. Genetics 1:355
    [Google Scholar]
  5. Fincham J. R. S. 1962; Genetically determined forms of glutamic dehydrogenase in Neurospora crassa . J. molec. Biol 4:257
    [Google Scholar]
  6. Grabar P., Williams C. A. 1955; Methode immunoélectrophoretique d’analyse des mélanges de substances antigéniques. Biochim. biophys. Acta 17:67
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr N. J., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  8. Markert C. L., ller F. 1959; Multiple forms of enzymes: tissue, ontogenetic, and species specific patterns. Proc. nat. Acad. Sci., Wash 45:753
    [Google Scholar]
  9. Ouchterloney Ö. 1953; Antigen antibody reactions in gel. Acta path, microbiol. scand 32:231
    [Google Scholar]
  10. Pateman J. A. 1959; The effect of selection on ascospore size in Neurospora crassa . Heredity 13:1
    [Google Scholar]
  11. Sanwal B. D., Lata M. 1962; The regulation of glutamic dehydrogenase and an antigenically related protein in amination deficient mutants of Neurospora. Archs. Biochem 98:420
    [Google Scholar]
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