1887

Abstract

SUMMARY: Reported sedimentation coefficients of bacterial ribosomes display noticeable variation. Since this might be due to differences in experimental conditions or to actual differences amongst the bacteria this problem needed re-investigation. The sedimentation coefficients of ribosomes from seven widely divergent bacteria (Streptomyces, Acetobacter, Pseudomonas, Azotobacter, Escherichia, Streptococcus and Bacillus) were determined by analytical ultracentrifugation. The bacteria were selected to cover nearly the entire range of molar guanine + cytosine content of DNA. Correction for pressure and dilution was always negligible; correction for temperature was eliminated by working directly at 20·0°. The correction for concentration was the most important one. Further reduction to standard conditions (from dilute buffer to water) resulted in a change of about 3%. The corrected sedimentation coefficients, expressed as ( , w)0, were nearly the same for all these bacteria, being in the ranges 29·5±2, 37·5±2·8, 56·3±1·9, 76·7±2·5, 110·5±1·6 × 10 sec. It thus seems unlikely that these coefficients will be useful as an aid in bacterial taxonomy. Disruption of samples of the same bacterial suspension by ultrasonic treatment or with the French pressure cell frequently resulted in quantitative, and sometimes qualitative, differences in the yield of ribosomes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-34-2-219
1964-02-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/34/2/mic-34-2-219.html?itemId=/content/journal/micro/10.1099/00221287-34-2-219&mimeType=html&fmt=ahah

References

  1. Bolton E. T., Britten R. J., Cowie D. B., McCarthy B. J., McQuillen K., Roberts R. B. 1959; Report of the Department of Terrestrial Magnetism. Yearb. Cameg. Instn 58:259
    [Google Scholar]
  2. Bowen T. J., Dagley S., Sykes J. 1959; A ribonucleoprotein component of Escherichia coli . Biochem. J 72:419
    [Google Scholar]
  3. Bowen T. J., Dagley S., Sykes J., Wild D. G. 1961; Nature of the ribosomes present in bacteria and yeast: a reappraisal. Nature, Lond 189:638
    [Google Scholar]
  4. Brown J. W., Rosenberg C. F. 1962; Sedimentation characteristics of cytoplasmic particles from Sarcina lutea . Biochim. biophys. Acta 61:657
    [Google Scholar]
  5. Dagley S., Sykes J. 1958; The influence of conditions of culture on certain soluble macromolecular components of Escherichia coli . Microsomal Particles and Protein Synthesis R. B. Roberts;62 New York: Pergamon Press:
    [Google Scholar]
  6. Ecker R. E., Schaechter M. 1963; Bacterial growth under conditions of limited nutrition. Ann. N.Y. Acad. Sci 102:549
    [Google Scholar]
  7. Elias H. 1961 Ultrazentrifugen-Methoden München: Beckman Instruments GmbH;
    [Google Scholar]
  8. Elson D. 1959; Preparation and properties of a ribonucleoprotein isolated from Escherichia coli . Biochim. biophys. Acta 36:362
    [Google Scholar]
  9. Gillchriest W. C., Bock R. M. 1958; Isolation and characterisation of bacterial nucleoprotein particles. Microsomal Particles and Protein Synthesis R. B. Roberts;1 New York: Pergamon Press:
    [Google Scholar]
  10. Huxley H. E., Zubay G. 1960; Electron microscope observations on the structure of microsomal particles from Escherichia coli . J. molec. Biol 2:10
    [Google Scholar]
  11. McCarthy B. J. 1960; Variations in bacterial ribosomes. Biochim. biophys. Acta 39:563
    [Google Scholar]
  12. Morowitz H. J., Tourtelotte M. E., Guild W. R., Castro E., Woese C. 1962; The chemical composition and submicroscopic morphology of Mycoplasma gallisepticum, avian PPLO 5969. J. molec. Biol 4:93
    [Google Scholar]
  13. Schachman H. K. 1957; Ultracentrifugation, diffusion and viscosimetry. Meth. Enzymol 4:32
    [Google Scholar]
  14. Schachman H. K., Pardee A. B., Stanier R. Y. 1952; Studies on macromolecular organisation of microbial cells. Archs. Biochem 38:245
    [Google Scholar]
  15. Svedberg T., Pedersen K. O. 1940 The Ultracentrifuge Oxford: Clarendon Press;
    [Google Scholar]
  16. Takai M., Oota Y., Osawa S. 1962; Isolation and some properties of 25-S ribosomes from E. coli . Biochim. biophys. Acta 55:202
    [Google Scholar]
  17. Tissières A., Watson J. D., Schlessinger D., Hollingworth B. R. 1959; Ribonucleoprotein particles from Escherichia coli . J. molec. Biol 1:221
    [Google Scholar]
  18. Woese C. R., Langridge R., Morowitz H. J. 1960; Microsome distribution during germination of bacterial spores. J. Bact 79:777
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-34-2-219
Loading
/content/journal/micro/10.1099/00221287-34-2-219
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error