1887

Abstract

SUMMARY: Spores of were gamma-irradiated (Co source) and viable counts performed by surface spread and tube dilution methods. Surface spread counts were greater than tube dilution counts by factors varying from 1·57 to 3·90 (aqueous suspensions) and 2·36 to 6·10 (freeze-dried). In 8 cases out of 10 there was no significant difference in the regression coefficients of log % survivors against radiation dose for the two counting methods under identical conditions of irradiation. Freeze-drying from 5 % (w/v) aqueous solutions of glucose, lactose or fructose had a significant protective effect on the radiation resistance of the spores. Freeze-drying from aqueous suspension, or from 5 % maltose had no effect on the resistance.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-34-2-185
1964-02-01
2024-10-09
Loading full text...

Full text loading...

/deliver/fulltext/micro/34/2/mic-34-2-185.html?itemId=/content/journal/micro/10.1099/00221287-34-2-185&mimeType=html&fmt=ahah

References

  1. Alexander P. 1959 Trans. IXth International Congress on Radiobiology 2966 B. Rajewsky; Stuttgart: Thieme:
    [Google Scholar]
  2. Bailey N. J. T. 1959 Statistical Methods in Biology London: English Universities Press;
    [Google Scholar]
  3. Barker S. A., Grant P. M., Stacey M., Ward R. B. 1959; Effects of gamma radiation. Part I. Polymer formation from sugars, hydroxy acids, and amino acids. J. chem. Soc2648
    [Google Scholar]
  4. Black S. A., Gerhardt P. 1961; Permeability of bacterial spores. I. Characterisation of glucose uptake. J. Bact 82:743
    [Google Scholar]
  5. Black S. A., Gerhardt P. 1962; Permeability of bacterial spores. IV. Water content, uptake, and distribution. J. Bact 83:960
    [Google Scholar]
  6. Cloutier J. A. R. 1961; Room-temperature stabilization of radiation-produced free radicals in barbituric acids. Can. J. Phys 39:514
    [Google Scholar]
  7. Cochran W. G. 1950; Estimation of bacterial densities by means of the ‘most probable number.’. Biometrics 6:105
    [Google Scholar]
  8. Collins M. A. 1962; Gamma irradiation damage in monosaccharides. Nature, Lond 193:1061
    [Google Scholar]
  9. Davis M. 1954; Irradiation of bacterial spores with low voltage electrons. Archs. Biochem 48:469
    [Google Scholar]
  10. Donnellan J. E., Morowitz H. J. 1957; The irradiation of dry spores of B. subtilis with fast charged particles. Radial. Res 7:71
    [Google Scholar]
  11. Finney D. J. 1951; The estimation of bacterial densities by dilution series. J. Hyg. Camb 49:26
    [Google Scholar]
  12. Fisher R. A., Yates F. 1957 Statistical Tables for Biological, Agricultural, and Medical Research 5th ed. London: Oliver and Boyd;
    [Google Scholar]
  13. Hill E., Phillips G. O. 1959; The inactivation of Bacillus subtilis spores in penicillin by gamma radiation. J. appl. Bact 22:8
    [Google Scholar]
  14. Hutchinson F. 1955; Use of charged particles to measure skin thickness and other surface properties. Ann. N.Y. Acad. Sci 59:494
    [Google Scholar]
  15. Hutchinson F. 1957; The distance that a radical formed by ionizing radiations can diffuse in a yeast cell. Radial. Res 7:473
    [Google Scholar]
  16. Khenokh M. A., .Kuzicheva E. A., Evdokimov V. F. 1961; The effect of gamma radiation of 60Co on dry carbohydrates. Dokl.obshchem. Sobran(English translation), 135, 262
    [Google Scholar]
  17. Moos W. S. 1952; Variation of irradiation effects on micro-organisms in relation to physical changes in their environment. J. Bad 63:688
    [Google Scholar]
  18. Phillips G. O., Moody G. J. 1959; The chemical action of gamma radiation on aqueous solutions of carbohydrates. Int. J. appl. Radiat 6:78
    [Google Scholar]
  19. Powers E. L., Webb R. B., Ehret C. F. 1960; Storage, transfer, and utilisation of energy from X-rays in bacterial spores. Radiat. Res Supplement 2:94
    [Google Scholar]
  20. Proctor B. E., Goldblith S. A., Oberle M., Miller W. C. 1955; Radiosensitivity of B. subtilis under different environmental conditions. Radiat. Res 3:295
    [Google Scholar]
  21. Roberts T. A. 1961 Studies on the antibacterial activity of gamma rays on spores of Bacillus subtilis Ph.D. Thesis, University of London:
    [Google Scholar]
  22. Tallentire A. 1958; An observed oxygen effect during gamma irradiation of dried bacterial spores. Nature, Lond 182:1024
    [Google Scholar]
  23. Tallentire A., Davies D. J. G. 1961; A post-irradiation oxygen effect in bacterial spores and its dependence on water content. Expl Cell Res 24:148
    [Google Scholar]
  24. Tallentire A., Powers E. L. 1961; The participation of bound water in the radiation response of bacterial spores. Radiat. Res 14:510 (Abstracts)
    [Google Scholar]
  25. Tallentire A., Powers E. L. 1963; Modification of sensitivity to X-irradiation by water in Bacillus magaterium . Radiat. Res 20:270
    [Google Scholar]
  26. Webb R. B., Powers E. L. 1961; Water, glycerol, and oxygen as factors in the radiation sensitivity of bacterial spores. Radiat. Res 14:515 (abstract)
    [Google Scholar]
  27. Woese C. R. 1958; Comparison of X-ray sensitivity of bacterial spores. J. Bact 75:5
    [Google Scholar]
  28. Wood T. H. 1959; Inhibition of cell division. Radiat. Res Supplement 1:332
    [Google Scholar]
/content/journal/micro/10.1099/00221287-34-2-185
Loading
/content/journal/micro/10.1099/00221287-34-2-185
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error