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Volume 34, Issue 1

Kinetic Studies of the Oxidative Assimilation of Acetate by a Non-photosynthetic Strain of Free

Abstract

SUMMARY: Kinetic studies of the effects of unlabelled acetate on CO production by a non-photosynthetic strain of C-labelled Euglena indicate the existence of a ‘labile reserve’, containing 1.5–2.0 μg.-atom C/million organisms, which is an early product of acetate assimilation and a substrate of endogenous metabolism. When this Euglena assimilated radioactive acetate, radioactivity in an ethanol-soluble form (Soluble I) increased rapidly at first, then levelled off at a value corresponding to about 0.2 μg-atom C/million organisms. Ethanol-insoluble radioactivity appeared slowly at first, then at an increasing rate which became linear at about 30–60 min. after addition of acetate. Kinetic analyses suggest that Soluble I is the precursor of the insoluble material. A second ethanol-soluble component began to become labelled some time after the first hour, and reached maximum radioactivity, corresponding to about 0.5 μg.-atom C/million organisms, 2–3 hr after addition of tracer acetate. The chemical composition of the ethanol-soluble and ethanol-insoluble components and their relationship to the ‘labile reserve’ are discussed.

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© Journal of General Microbiology 1963
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1964-01-01
2024-10-12
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References

  1. Barker S. A., Bourne E. J. 1955 Biochemistry and Physiology of Protozoa Ed. by ii45 Hutner S. H., Lwoff A. New York: Academic Press Inc;
     [Google Scholar]
  2. Bevington J. C., Bourne E. J., Turton C. N. 1953; Chemical degradation of 14C-glucose and its application to 14C-starch from Polytomella coeca . Chem. & Ind1390
     [Google Scholar]
  3. Buetow D. E. 1961; Variation of the respiration of protozoan cells with length of centrifuging. Analyt. Biochem 2:242
     [Google Scholar]
  4. Danforth W. 1953; Oxidative metabolism of Euglena. Arch. Biochem 46:164
     [Google Scholar]
  5. Danforth W. F. 1961; Oxidative assimilation of acetate by Euglena. Carbon balance and effects of ethanol. J. Protozool 8:152
     [Google Scholar]
  6. Danforth W. F., Wilson B. W. 1957; Adaptive changes in the acetate metabolism of Euglena. J. Protozool 4:52
     [Google Scholar]
  7. Danforth W. F., Wilson B. W. 1961; The endogenous metabolism of Euglena gracilis . J. gen. Microbiol 24:95
     [Google Scholar]
  8. Lynch V. H., Calvin M. 1952; Carbon dioxide fixation by microorganisms. J. Bact 63:525
     [Google Scholar]
  9. Lynch V. H., Calvin M. 1953; CO2 fixation by Euglena. Ann. N.Y. Acad. Sci 56:890
     [Google Scholar]
  10. Marzullo G., Danforth W. F. 1964; Composition of ethanol-insoluble assimilatory products of oxidative assimilation of acetate by Euglena gracilis . J. gen. Microbiol 34:21
     [Google Scholar]
  11. Wilson B. W., Danforth W. F. 1958; The extent of acetate and ethanol oxidation by Euglena gracilis . J. gen. Microbiol 18:535
     [Google Scholar]
/content/journal/micro/10.1099/00221287-34-1-9
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Kinetic Studies of the Oxidative Assimilation of Acetate by a Non-photosynthetic Strain of Euglena gracilis
Microbiology 34, 9 (1964); https://doi.org/10.1099/00221287-34-1-9
/content/journal/micro/10.1099/00221287-34-1-9
/content/journal/micro/10.1099/00221287-34-1-9
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