1887

Abstract

SUMMARY: After incubation for 6 hr. with uniformly labelled C-acetate, about 90% of the acetate carbon assimilated by non-photosynthetic var. was in the form of cellular materials insoluble in 60% (v/v) ethanol in water. Within this fraction about 80% of the assimilated carbon was in the form of glucose units of the polysaccharide paramylon, about 9% was in a fraction containing proteins, nucleic acids and probably other materials, and about 2% was in lipid. The ratio of assimilated carbon to total carbon in the paramylon was 3 to 5 times that in the protein + nucleic acid or lipid fractions.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-34-1-21
1964-01-01
2024-12-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/34/1/mic-34-1-21.html?itemId=/content/journal/micro/10.1099/00221287-34-1-21&mimeType=html&fmt=ahah

References

  1. Clarke A. E., Stone B. A. 1960; Structure of the paramylon from Euglena gracilis . Biochim. biophys. Acta 44:161
    [Google Scholar]
  2. Cramer M., Myers J. 1949; Effects of starvation on the metabolism of Chlorella. Plant Physiol 24:255
    [Google Scholar]
  3. Danforth W. F. 1961; Oxidative assimilation of acetate by Euglena. Carbon balance and effects of ethanol. J. Protozool 8:52
    [Google Scholar]
  4. Danforth W. F., Wilson B. W. 1957; Adaptive changes in the acetate metabolism of Euglena. J. Protozool 4:52
    [Google Scholar]
  5. Danforth W. F., Wilson B. W. 1961; The endogenous metabolism of Euglena gracilis . J. gen. Microbiol 24:95
    [Google Scholar]
  6. Johnson M. J. 1941; Isolation and properties of a pure yeast polypeptidase. J. biol. Chem 137:575
    [Google Scholar]
  7. Marzullo G., Danforth W. F. 1964; Kinetic studies of the oxidative assimilaton of acetate by a non-photosynthetic strain of Euglena gracilis . J. gen. Microbiol 34:9
    [Google Scholar]
  8. Mokrasch L. C. 1954; Analysis of the hexose phosphate and sugar mixture with the anthrone reagent. J. biol. Chem 208:55
    [Google Scholar]
  9. Myers J., Cramer M. 1948; Metabolic conditions in Chlorella. J. gen. Physiol 32:103
    [Google Scholar]
  10. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. J. biol. Chem 153:375
    [Google Scholar]
  11. Trevelyan W. E., Harrison J. S. 1952; Fractionation and micro-determination of all carbohydrate. Biochem. J 50:298
    [Google Scholar]
  12. Wilson B. W. 1963; Controlled growth and the regulation of the metabolism of the flagellates Astasia and Euglena. Thesis, University of California, Los Angeles:
    [Google Scholar]
  13. Wilson B. W., Danforth W. F. 1958; The extent of acetate and ethanol oxidation by Euglena gracilis . J. gen. Microbiol 18:535
    [Google Scholar]
  14. von Witsch H. 1948; PhysiologischerZustand und Wachstumsintensitatbei Chlorella. Arch. Mikrobiol 14:128
    [Google Scholar]
/content/journal/micro/10.1099/00221287-34-1-21
Loading
/content/journal/micro/10.1099/00221287-34-1-21
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error