1887

Abstract

SUMMARY: Samples of purified vaccinia virus chemically analysed had the following composition (%): N, 14.7; P, 0.49; S, 0.76; DNA, 3.2; total lipid, 5.0 (including cholesterol, 1.2; phospholipid, 2.1); trace constituents detected were: RNA, 0.1; carbohydrate, 0.2; Cu, 0.02; flavin, 5 × 10; biotin, 1.3 × 10. These results are compared with other published figures and the differences discussed. The trace constituents are not considered to be true components of the virus. On the other hand, there is no evidence for regarding cholesterol and the other lipid components as non-essential constituents of the virus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-34-1-115
1964-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/34/1/mic-34-1-115.html?itemId=/content/journal/micro/10.1099/00221287-34-1-115&mimeType=html&fmt=ahah

References

  1. Allison A. C., Burke D. C. 1962; The nucleic acid contents of viruses. J. gen. Microbiol 27:181
    [Google Scholar]
  2. Andrewes C. H., Horstmann D. M. 1949; The susceptibility of viruses to ethyl ether. J. gen. Microbiol 3:290
    [Google Scholar]
  3. Bessey O. A., Lowry O. H., Love R. H. 1949; The fluorimetric measurement of the nucleotides of riboflavin and their concentration in tissues. J. biol. Chem 180:755
    [Google Scholar]
  4. Bolognani L., Coppi G., Zambotti Y. 1961; Determination of pentoses. Experientia 17:67
    [Google Scholar]
  5. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J 62:315
    [Google Scholar]
  6. Green B., Lowe J. S., Morton R. A. 1955; The effect of vitamin A deficiency on the cholesterol levels of the plasma and liver of the rat. Biochem. J 61:447
    [Google Scholar]
  7. Hoagland C. L., Lavin G. I., Smadel J. E., Rivers T. M. 1940; Constituents of elementary bodies of vaccinia. II. Properties of nucleic acid obtained from vaccine virus. J. exp. Med 72:139
    [Google Scholar]
  8. Hoagland C. L., Smadel J. E., Rivers T. M. 1940; Constituents of elementary bodies of vaccinia. I. Certain basic analyses and observations on lipid components of the virus. J. exp. Med 71:737
    [Google Scholar]
  9. Hoagland C. L., Ward S. M., Smadel J. E., Rivers T. M. 1940; Biotin in elementary bodies of vaccinia. Proc. Soc. exp. Biol., N.Y 45:669
    [Google Scholar]
  10. Hoagland C. L., Ward S. M., Smadel J. E., Rivers T. M. 1941; Constituents of elementary bodies of vaccinia. IV. Demonstration of copper in the purified virus. J. exp. Med 74:69
    [Google Scholar]
  11. Joklik W. K. 1962a; The purification of four strains of poxvirus. Virology 18:9
    [Google Scholar]
  12. Joklik W. K. 1962b; The preparation and characteristics of highly purified radioactively labelled poxvirus. Biochim. biophys. Acta 61:290
    [Google Scholar]
  13. kabat E. A., Mayer M. M. 1948 Experimental Immunochemistry 1st ed 315 Springfield, Illinois: Charles C. Thomas;
    [Google Scholar]
  14. King E. J. 1946 Microanalysis in Medical Biochemistry 1st ed., 51 London: Churchill;
    [Google Scholar]
  15. Kolthoff I. M., Sandell E. B. 1952 Textbook of Quantitative Inorganic Analysis 3rd ed., 637 New York: Macmillan;
    [Google Scholar]
  16. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  17. Planterose D. N., Nishimura C., Salzman N. P. 1962; The purification of vaccinia virus from cell cultures. Virology 18:294
    [Google Scholar]
  18. Schmidt G., Thannhauser S. J. 1945; A method for the determination of desoxyribonucleic acid, ribonucleic acid and phosphoproteins in animal tissues. J. biol. Chem 161:83
    [Google Scholar]
  19. Smadel J. E., Hoagland C. L. 1942; Elementary bodies of vaccinia. Bact. Rev 6:79
    [Google Scholar]
  20. Smadel J. E., Rivers T. M., Hoagland C. L. 1942; Nucleoprotein antigen of vaccine virus. I. A new antigen obtained from elementary bodies of vaccinia. Arch. Path 34:275
    [Google Scholar]
  21. Spencer B. 1960; The ultramicro determination of inorganic sulphur. Biochem. J 75:435
    [Google Scholar]
  22. Westwood J. C. N., Phipps P. H., Boulter E. A. 1957; The titration of vaccinia virus on the chorioallantoic membrane of the developing chick embryo. J. Hyg., Camb 55:123
    [Google Scholar]
  23. Wright L. D., Skeggs H. R. 1944; Determination of biotin with Lactobacillus arabinosus . Proc. Soc. exp. Biol., N.Y 56:95
    [Google Scholar]
  24. Wyatt G. R., Cohen S. S. 1953; The bases of the nucleic acids of some bacterial and animal viruses: The occurrence of 5-hydroxymethyl-cytosine. Biochem. J 55:774
    [Google Scholar]
  25. Zwartouw H. T., Westwood J. C. N., Appleyard G. 1962; Purification of poxviruses by density gradient centrifugation. J. gen. Microbiol 29:523
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-34-1-115
Loading
/content/journal/micro/10.1099/00221287-34-1-115
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error