1887

Abstract

SUMMARY: Cell walls of which were treated with trypsin, ‘Pronase’ (a protease from ), or lysozyme were more serologically reactive than cell walls treated with ribonuclease, pepsin or lipase or those with no enzymic treatment. Ribonuclease decreased reactivity of cell wall suspensions. Electron microscopy revealed the presence of round ‘plates’ following treatment with certain of these enzymes. ‘Plates’ were also shown to be embedded in fragments of the cell wall following lysozyme treatment. Still smaller round bodies were also seen to be present. These particles are believed to be a sub-unit of cell walls and are correlated with serologic reactivity.

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/content/journal/micro/10.1099/00221287-34-1-1
1964-01-01
2022-01-18
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References

  1. Foster J. W., Cowan R. M., Maag T. A. 1962; Rupture of bacteria by explosive decompression. J. Bact 83:330
    [Google Scholar]
  2. Foster J. W., Ribi E. 1960; The role of the cell wall of Brucellaabortus in immunity. Bact. Proc
    [Google Scholar]
  3. Foster J. W., Ribi E. 1962; The role of the cell wall of Brucellaabortus in immunity. J. Bact 84:258
    [Google Scholar]
  4. Houwink A. L. 1953; A macromolecular mono-layer in the cell wall of Spirillum sp. Biochim. biophys. acta 10:360
    [Google Scholar]
  5. Larson C. L. 1951; Studies on thermostable antigens extracted from Bacterium tularense and from tissues of animals dead of tularemia. J. Immunol 66:249
    [Google Scholar]
  6. Markenson J., Sulitzeanu D., Olitzki A. 1959; Immunizing properties of insoluble cell material derived from Brucella. Nature, Lond 183:1693
    [Google Scholar]
  7. Markenson J., Sulitzeanu D., Olitzki A. 1962; Immunogenic activity of Brucella cell wall. Brit. J. exp. Path 39:219
    [Google Scholar]
  8. Miles A. A., Pirie N. W. 1939; The properties of antigenic preparations from Brucellamelitensis. II. Serological properties of the antigens. Brit. J. exp. Path 20:109
    [Google Scholar]
  9. Olitzki A. L. 1960; The antigenic relationship between phenol extracted bacterial deoxyribonucleic acid and other soluble antigens of brucellae studied with the aid of the agar-gel precipitation technique. Brit. J. exp. Path 41:623
    [Google Scholar]
  10. Olitzki A. L., Sulitzeanu D. 1958; Studies on the antigenic structure of Brucellasuis with the aid of agar-gel precipitation technique. Brit. J. exp. Path 39:219
    [Google Scholar]
  11. Ribi E., Milner K. C., Perrine T. D. 1959; Endotoxic and antigenic fractions from the cell wall of Salmonella enteritidis . J. Immunol 82:75
    [Google Scholar]
  12. Salton M. R. S. 1960 Microbial Cell Walls. Ciba Lectures in Microbial Biochemistry New York: John Wiley and Sons Inc;
    [Google Scholar]
  13. Shepard C. S., Ribi E., Larson C. L. 1954; Electron microscopically revealed structural elements of Bacterium tularensis and their in vitro and in vivo role in immunologic reactions. J. Immunol 75:7
    [Google Scholar]
  14. Weidel W., Frank H., Martin H. H. 1960; The rigid layer of the cell wall of Escherichia coli strain b . J. gen. Microbiol 22:185
    [Google Scholar]
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