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Volume 33, Issue 3

Chemical Composition of Mycoplasma Cells and Membranes Free

Abstract

SUMMARY: The chemical composition of whole organisms and cell membranes of eight saprophytic and parasitic strains was studied. Cell membranes of and were prepared by osmotic shock. The membranes comprised about 35 % of the dry weight of the organisms. Generally the solid residue of the mycoplasmas contained 54–62 % protein, 12–20 % lipid, 3–8 % carbohydrate, 8–17 % RNA and 4–7 % DNA. Cell membranes contained 47–60 % protein, 35–37 % lipid, 4–7 % carbohydrate, and small amounts of RNA and DNA which could not be washed away with de-ionized water. The saprophytic contained only one-fourth to one-third of the total cholesterol found in the lipid of the parasitic strains. No cholesterol was detected in grown in cholesterol-free media. Acetone-insoluble lipids formed the major part of the lipid in the saprophytic strains, and the minor part in the lipid of the parasitic strains. The carotenoid pigments of were retained in the cell membrane preparations.

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© Journal of General Microbiology 1963
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1963-12-01
2024-04-16
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References

  1. Al-Doory Y., Larsh H. W. 1962; Quantitative studies of total lipids of pathogenic fungi.. Appl. Microbiol 10492
     [Google Scholar]
  2. Avigan J., Goodman D. S., Steinberg D. 1963; Thin-layer chromatography of sterols and steroids.. J. Lipid Res 4100
     [Google Scholar]
  3. Burgen A. S. V. 1962; The structure and function of cell membranes.. Canad. J. Biochem. Physiol 401253
     [Google Scholar]
  4. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.. Biochem. J 62315
     [Google Scholar]
  5. Buttery S. H., Plackett P. 1960; A specific polysaccharide from Mycoplasma mycoides.. J. gen. Microbiol 23357
     [Google Scholar]
  6. Chen C. C., Cleverdon R. C. 1962; The chemical composition of Dialister pneumosintes and the significance of the studies of small eubacteria.. Life Sciences 1401
     [Google Scholar]
  7. DeLey J. 1962; The separation of ribosomes and oxidosomes from bacteria.. Biochem. J 849
     [Google Scholar]
  8. Drury H. F. 1948; Identification and estimation of pentoses in the presence of glucose.. Arch. Biochem 19455
     [Google Scholar]
  9. Dubois M., Gilles K., Hamilton K., Rebas P., Smith E. 1956; Determination of carbohydrates by phenol.. Anal. Chem 28350
     [Google Scholar]
  10. Edward D. G. ff. 1953; A difference in growth requirements between bacteria in the L-phase and organisms of the pleuro-pneumonia group.. J. gen. Microbiol 8256
     [Google Scholar]
  11. Fiertel A., Klein H. P. 1959; On sterols in bacteria.. J. Bact 78738
     [Google Scholar]
  12. Folch J., Lees M., Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues.. J. biol. Chem 226497
     [Google Scholar]
  13. Freimer E. H. 1963; Studies of L forms and protoplasts of group A streptococci II. Chemical and immunological properties of the cell membrane.. J. exp. Med 117377
     [Google Scholar]
  14. Gilby A. R., Few A. V., McQuillen K. 1958; The chemical composition of the protoplast membrane of Micrococcus lysodeikticus.. Biochim. biophys. Acta 2921
     [Google Scholar]
  15. Hughes D. E. 1962; The bacterial cytoplasmic membrane.. J. gen. Microbiol 2939
     [Google Scholar]
  16. Hunter G. D., Godson G. N. 1962; The final stages of protein synthesis and the role of lipids in the process.. J. gen. Microbiol 2965
     [Google Scholar]
  17. Ikawa M. 1963; Nature of the lipids of some lactic acid bacteria.. J. Bact 85772
     [Google Scholar]
  18. Kandler O., Zehender C. 1957; über das Vorkommen von α, ॉ-Diaminopimelinsäure bei verschiedenen L-Phasentypen von Proteus vulgaris und bei den pleuropneumonie-ähnlichen Organismen.. Z. Naturf 12b725
     [Google Scholar]
  19. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent.. J. biol. Chem 193265
     [Google Scholar]
  20. Lucy J. A., Dingle J. T. 1962; Vitamin A and membrane systems.2. Membrane stability and protein-vitamin A-lipid interactions.. Biochem. J 8476
     [Google Scholar]
  21. Lynn R. J., Smith P. F. 1957; Nucleic acid content of pleuropneumonia-like organisms from human sources.. J. Bact 74811
     [Google Scholar]
  22. Lynn R. J., Smith P. F. 1960; Chemical composition of PPLO.. Ann. N.Y. Acad. Sci 79493
     [Google Scholar]
  23. MacFarlane M. G. 1961; Composition of lipid from the protoplast membranes and whole cells of Micrococcus lysodeikticus.. Biochem. J 794
     [Google Scholar]
  24. MacLeod P., Brown J. P. 1963; Fatty acid composition of lipids from Streptococcus cremoris and Streptococcus lactis var. maltigenes.. J. Bact 851056
     [Google Scholar]
  25. Markovitz A., Dorfman A. 1962; Synthesis of capsular polysaccharide (hyaluronic acid) by protoplast membrane preparations of group A streptococcus.. J. biol. Chem 237273
     [Google Scholar]
  26. Mitchell P. 1959; Structure and function in microorganisms.. Biochem,. Soc. Symp 1673
     [Google Scholar]
  27. Morowitz H. J., Cleverdon R. C. 1959; An extreme example of the coding problem, avian PPLO 5969.. Biochim. biophys. Acta 34578
     [Google Scholar]
  28. Morowitz H. J., Cleverdon R. C. 1960; The irradiation of the pleuropneumonia-like organism A 5969 with cobalt-60 gamma rays.. Radiation Res 13854
     [Google Scholar]
  29. Morowitz H. J., Tourtellotte M. E., Guild W. R., Castro E., Woese C., Cleverdon R. C. 1962; The chemical composition and sub-microscopic morphology of Mycoplasma gallisepticum, avian PPLO 5969.. J. molec. Biol 493
     [Google Scholar]
  30. O’Leary W. M. 1962; The fatty acids of bacteria.. Bact. Rev 26421
     [Google Scholar]
  31. Panos C., Barkulis S. S., Hayashi J. A. 1959; Streptococcal L forms. II. Chemical composition.. J. Bact 78863
     [Google Scholar]
  32. Plackett P. 1959; On the absence of ‘mucocomplex’ from Mycoplasma mycoides.. Biochim. Mophys. Acta 35260
     [Google Scholar]
  33. Plackett P. 1961; A polyglycerophosphate compound from Mycoplasma mycoides.. Nature, Lond 189125
     [Google Scholar]
  34. Razin S. 1963; Structure, composition and properties of the PPLO cell envelope.. Recent Progress in Microbiology, VIII p. 526. Ed. by Gibbons N. E. Toronto: University Press;
     [Google Scholar]
  35. Razin S. 1964; Osmotic lysis of Mycoplasma.. J. gen. Microbiol 33471
     [Google Scholar]
  36. Razin S., Argaman M. 1961; Properties of the Mycoplasma (PPLO) cell envelope.. Bull. Res. Conn. Israel 9e 121
     [Google Scholar]
  37. Razin S., Argaman M. 1963; Lysis of Mycoplasma, bacterial protoplasts, spheroplasts and L-forms by various agents.. J. gen. Microbiol 30155
     [Google Scholar]
  38. Razin S., Cohen A. 1963; Nutritional requirements and metabolism of Mycoplasma laidlawii.. J. gen. Microbiol 30141
     [Google Scholar]
  39. Razin S., Michmann J., Shimshoni Z. 1964; The occurrence of Mycoplasma (pleuropneumonia-like organisms, PPLO) in the oral cavity of dentulous and edentulous subjects.. J. dent. Res (in the Press)
    [Google Scholar]
  40. Razin S., Rottem S. 1964; Fatty acid requirements of Mycoplasma laidlawii.. J. gen. Microbiol 33459
     [Google Scholar]
  41. Robertson J. D. 1959; The ultrastructure of cell membranes and their derivatives.. Biochem. Soc. Symp 163
     [Google Scholar]
  42. Rothblat G. H., Smith P. F. 1961; Nonsaponifiable lipids of representative pleuropneumonia-like organisms.. J. Bact 82479
     [Google Scholar]
  43. Schneider W. C. 1945; Phosphorus compounds in animal tissues. I. Extraction and estimation of desoxypentose nucleic acid and of pentose nucleic acid.. J. biol. Chem 161293
     [Google Scholar]
  44. Schwan H. P., Morowitz H. J. 1962; Electrical properties of the membranes of the pleuropneumonia-like organism A 5969.. Biophysical J 2395
     [Google Scholar]
  45. Shockman G. D., Kolb J. J., Bakay B., Conover M. J., Toennies G. 1963; Protoplast membrane of Streptococcus faecalis.. J. Bact 85168
     [Google Scholar]
  46. Smith P. F., Lecce J. G., Lynn R. J. 1954; A lipoprotein as a growth factor for certain pleuropneumonia-like organisms.. J. Bact 68627
     [Google Scholar]
  47. Smith P. F., Rothblat H. G. 1960; Incorporation of cholesterol by pleuropneumonialike organisms.. J. Bact 80842
     [Google Scholar]
  48. Smith P. F., Rothblat G. H. 1962; Comparison of lipid composition of pleuropneumonia-like and L-type organisms.. J. Bact 83500
     [Google Scholar]
  49. Suit J. C. 1963; Ribonucleic acid in a ‘membrane’ fraction of Escherichia coli and its relation to cell-wall synthesis.. J. Bact 841061
     [Google Scholar]
  50. Umbreit W. W., Burris R. H., Stauffer J. F. 1957 Manometric Techniques Minneapolis: Burgess Publishing Co;
     [Google Scholar]
  51. VanIterson W., Ruys A. C. 1960; The fine structure of the Mycoplasmataceae (micro-organisms of the pleuropneumonia group PPLO).1. Mycoplasmahominis, M. fermentans and M. salivarium.. J. Ultrastr. Res 3282
     [Google Scholar]
  52. Vendrely R., Tulasne R. 1953; Chemical constitution of the L-forms of bacteria.. Nature, Land 171262
     [Google Scholar]
  53. Weibull C., m L. 1958; The chemical nature of the cytoplasmic membrane and cell wall of Bacillus megaterium, strain M.. Biochim. biophys. Acta 30340
     [Google Scholar]
  54. Weibull C., Beckman H., m L. 1959; Localization of enzymes in Bacillus megaterium strain M.. J. gen. Microbiol 20519
     [Google Scholar]
  55. Wycoff H. D., Parsons J. 1957; Chromatographic microassay for cholesterol and cholesterol esters.. Science 125347
     [Google Scholar]
  56. Yudkin M. D. 1962; Chemical studies of the protoplast membrane of Bacillus megaterium KM.. Biochem. J 8240
     [Google Scholar]
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Chemical Composition of Mycoplasma Cells and Membranes
Microbiology 33, 477 (1963); https://doi.org/10.1099/00221287-33-3-477
/content/journal/micro/10.1099/00221287-33-3-477
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