SUMMARY: Several strains of were grown in a partially defined medium which contained bovine plasma albumin as the only undefined constituent. Lipid extraction of the albumin abolished its growth-promoting activity. Growth was restored by adding the extracted lipid fraction or by long-chain fatty acids. Of the fatty acids tested oleic acid was the most active in growth promotion; linoleic and linolenic acids were less active; palmitic and stearic acids were inactive. The requirement for oleic acid was satisfied by Tween 80. was found to split Tween 80 enzymically, liberating free oleic acid. Replacement of bovine albumin by certain degradation products and by oleic acid, methyl oleate or Tween 80 was unsuccessful, nor could bovine albumin be replaced by several polymers known to bind fatty acids or to increase the viscosity of the growth medium.


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