SUMMARY: The rumen oligotrich protozoa, Eremoplastron bovis (Dogiel, 1927), E. brevispinum (Kofoid & MacLennan, 1932), Entodinium longinucleatum (Dogiel, 1925), E. ovinum (Dogiel, 1927), Eudiplodinium maggii (Fiorentini, 1889) and various Epidinium species have been cultured in mixed culture for over 7 months. The medium consisted of rice starch, dried grass and casein in a mineral salt solution + rumen liquor. No antibiotics were used. Preliminary studies showed that sodium sulphide causes inhibition of protozoal metabolism in concentrations greater than 0·01 %. Pure cultures of Epidinium ecaudatum (Fiorentini, 1889) and Eremoplastron bovis have been established. In addition, a clone of E. bovis was maintained for 5 months. A method for growing quantities of protozoa for biochemical studies is described.
KandatsuM.,
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KofoidC. A.,
MaclennanR. F.1933; Ciliates from Bos indicus Linn. III. Epidinium Crawley, Epiplastron gen. nov., and Ophryoscolex Stein.. Univ. Calif. Publ. Zool391
OxfordA. E.1958; Bloat in cattle. IX. Some observations on the culture of the cattle rumen ciliate Epidinium ecaudatum Crawley occurring in quantity in cows fed on red clover (Trifolium pratense L.). N.Z.. J. agric. Res1809