1887

Abstract

SUMMARY: The sodium azide resistance of is not of the all-or-none unilocal type reported for but rather of the obligatory multi-step or penicillin variety. Independently isolated 1st-step resistant variants possessed similar degrees of resistance to sodium azide. Some properties of azide-resistant variants of are described. In support of the above finding it was possible to transduce 1st-step (and only 1st-step) resistance into the wild-tpye by phage grown on either 1st-, 2nd-, 3rd- or 4th-step resistant organisms. It was also possible to transduce 2nd-step resistance into 1st-step organisms by phage developed on independently isolated 1st step resistant organisms or by phage from multi-step resistant. About 60 % of transduced genes expressed their phenotype in platings done immediately after the adsorption period. It is concluded that a number of loci, not closely linked, and possibly equipotent, control sodium azide resistance in and that resistance could be dominant to the wild allele.

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1963-11-01
2024-04-23
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References

  1. Banič, S 1959; Transduction to penicillin and chloramphenicol resistance in Salmonella typhimurium.. Genetics 44:449
    [Google Scholar]
  2. Bryson V., Demerec M. 1950; Patterns of resistance to antimicrobial agents.. Ann. N.Y. Acad. Sci 53:283
    [Google Scholar]
  3. Bryson V., Szybalski W. 1955; Microbial drug resistance. Advanc.. Genet 7:1
    [Google Scholar]
  4. Cavalli L. L. 1952; Genetic analysis of drug-resistance.. Bull. Wld Hlth Org 6:185
    [Google Scholar]
  5. Cavalli L. L., Maccacaro G. A. 1950; Chloromycetin resistance in Escherichia coli, a case of quantitative inheritance in bacteria.. Nature, Lond 166:991
    [Google Scholar]
  6. Cavalli L. L., Maccacaro G. A. 1952; Polygenic inheritance of drug-resistance in the bacterium Escherichia coli.. Heredity 6:311
    [Google Scholar]
  7. Coetzee J. N. 1961; Lysogenic conversion in the genus Proteus.. Nature, Lond 189:946
    [Google Scholar]
  8. Coetzee J. N. 1963; Transduction of swarming in Proteus mirabilis.. J. gen. Microbiol 33:1
    [Google Scholar]
  9. Coetzee J. N., Sacks T. G. 1960a; Transduction of streptomycin resistance in Proteus mirabilis.. J. gen. Microbiol 23:445
    [Google Scholar]
  10. Coetzee J. N., Sacks T. G. 1960b; Morphological variants of Proteus hauseri.. J. gen. Microbiol 23:209
    [Google Scholar]
  11. Davis G. H. G. 1955; The classification of Lactobacilli from the human mouth.. J. gen. Microbiol 13:481
    [Google Scholar]
  12. Demerec M. 1945; Production of staphylococcus strains resistant to various concentrations of penicillin.. Proc. nat. Acad. Sci., Wash 31:16
    [Google Scholar]
  13. Demerec M. 1948; Origin of bacterial resistance to antibiotics.. J. Bact 56:63
    [Google Scholar]
  14. Demerec M. 1951; Studies of the streptomycin-resistance system of mutations in Escherichia coli.. Genetics 36:585
    [Google Scholar]
  15. Diamond B. E. 1950; A selective medium for lactobacilli counts from saliva.. J. dent. Res 29:8
    [Google Scholar]
  16. Edgar J. B., Stocker B. A. D. 1961; Metabolic and genetic investigations of nutritionally exacting strains of Staphylococcus pyogenes.. Nature, Lond 191:1121
    [Google Scholar]
  17. Forget A., Fredette V. 1962; Sodium azide selective medium for the primary isolation of anaerobic bacteria.. J. Bact 83:1217
    [Google Scholar]
  18. Gerencser V. F., Weaver R. H. 1959; A new technique for the use of sodium azide (Hydrazoic acid) as an inhibitive agent.. Appl. Microbiol 7:113
    [Google Scholar]
  19. Hayes W. 1957; The kinetics of the mating process in Escherichia coli.. J. gen. Microbiol 16:97
    [Google Scholar]
  20. Holloway B. W., Monk M. 1959; Transduction in Pseudomonas aeruginosa.. Nature, Lond 184:1426
    [Google Scholar]
  21. Hotchkiss R. D. 1951; Transfer of penicillin resistance in pneumococci by the desoxyribonucleate derived from resistant cultures.. Cold Spr. Harb. Symp. quant. Biol 16:457
    [Google Scholar]
  22. Hotchkiss R. D. 1952; The role of desoxyribonucleates in bacterial transformations.. In Phosphorus Metabolism Vol ii p 426 Ed McElroy W. D., Glass B. Baltimore: The Johns Hopkins Press;
    [Google Scholar]
  23. Jacob F., Wollman E. L. 1961 Sexuality and the Genetics of Bacteria London: Academic Press;
    [Google Scholar]
  24. Kauffmann F. 1951 Enterobaderiaceae Copenhagen: Einar Munksgaard;
    [Google Scholar]
  25. Keilin D., Hartree E. F. 1934; Inhibitors of catalase reaction.. Nature, Lond 134:933
    [Google Scholar]
  26. Lederberg J. 1947; Gene recombination and linked segregations in Escherichia coli.. Genetics 32:505
    [Google Scholar]
  27. Lederberg J. 1950; The selection of genetic recombinationswith bacterial growth inhibitors.. J. Bad 59:211
    [Google Scholar]
  28. Lederberg J. 1951; Streptomycin resistance: a genetically recessive mutation.. J. Bact 75:11
    [Google Scholar]
  29. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1:190
    [Google Scholar]
  30. Lichstein H. C., Soule M. H. 1944a; Studies of the effect of sodium azide on microbic growth and respiration. I. The action of sodium azide on microbic growth.. J. Bact 47:221
    [Google Scholar]
  31. Lichstein H. C., Soule M. H. 1944b; Studies of the effect of sodium azide on microbic growth and respiration. II. The action of sodium azide on bacterial catalase.. J. Bact 47:231
    [Google Scholar]
  32. Loew O. 1891; Ueber das Verhalten des Aziomidszulebenden Organismen.. Ber. dtsch. chem. Ges 24:2947
    [Google Scholar]
  33. Mackie and McCartney’s Handbook of Bacteriology 1960 10th ed. Ed. by Cruickshank R. Edinburgh: E. and S. Livingstone;
  34. Manten A., Rowley D. 1953; Genetic analysis of valine inhibition in the K12 strain of Bacterium coli.. J. gen. Microbiol 9:226
    [Google Scholar]
  35. Mayr-Harting A. 1955; The acquisition of penicillin resistance by Staphylococcus aureusstrain Oxford.. J. gen. Microbiol 13:9
    [Google Scholar]
  36. Mitchison D. A. 1953; The occurrence of independent mutations to different types of streptomycin resistance in Bacterium coli.. J. gen. Microbiol 8:168
    [Google Scholar]
  37. Morse M. L. 1959; Transduction by staphylococcal bacteriophage.. Proc. nat. Acad. Sci., Wash 45:722
    [Google Scholar]
  38. Ozeki H. 1956; Abortive transduction in purine-requiring mutants of Salmonella typhimurium. Genetic studies with bacteria.. Publ. Carneg. Instn 612:97
    [Google Scholar]
  39. Pike R. M. 1944; An enrichment broth for isolating streptococci from throat swabs.. Proc. Soc. exp. Biol., N.Y 57:186
    [Google Scholar]
  40. Schäfer W., Haas F. 1957; Über die Wirkung von Natriumazid auf die Bakterienatmung.. Zbl. Bakt. (Abt. 1 Orig.) 169:402
    [Google Scholar]
  41. Sevag M. G., Shelbourne M. 1942; Cyanide-sensitive bacterial respiratory systems different from the usual cytochrome-cytochrome oxidase system.. J. gen. Physiol 26:1
    [Google Scholar]
  42. Snyder M. L., Lichstein H. C. 1940; Sodium azide as an inhibitory substance for Gram-negative bacteria.. J. infect. Dis 67:113
    [Google Scholar]
  43. Stocker B. A. D. 1956; Abortive transduction of motility in Salmonella; a non-replicated gene transmitted through many generations to a single descendant.. J. gen. Microbiol 15:575
    [Google Scholar]
  44. Szybalski W., Bryson V. 1954; Genetic studies on microbial cross resistance to toxic agents. III. Cross resistance of Mycobacterium ranae to twenty-eight antimicrobial agents.. Amer. rev. Tuberc 69:267
    [Google Scholar]
  45. Thorne C. B. 1962; Transduction in Bacillus subtilis.. J. Bad 83:106
    [Google Scholar]
  46. Thornley M. J., Yudkin J. 1959; The origin of bacterial resistance to proflavine. 2. Spontaneous mutation to proflavine resistance in Escherichia coli.. J. gen. Microbiol 20:365
    [Google Scholar]
  47. Watanabe T. 1954; Genetic studies on the mechanisms of acquired streptomycin resistance in microorganisms.. Keio J. Med 3:193
    [Google Scholar]
  48. Watanabe T., Watanabe M. 1959a; Transduction of streptomycin resistance in Salmonella typhimurium.. J. gen. Microbiol 21:16
    [Google Scholar]
  49. Watanabe T., Watanabe M. 1959b; Transduction of streptomycin sensitivity into resistant mutants of Salmonella typhimurium.. J. gen. Microbiol 21:30
    [Google Scholar]
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