1887

Abstract

SUMMARY: A method is described for assaying flagella of based on the incorporation of a C-labelled amino acid, mechanical separation of the flagella from the bacteria, and measurement of the radioactivity of the isolated flagella. There is no detectable lag before the appearance of L-[(-H]leucine, or L-[-C]methionine in the flagella, and the rates of incorporation of these amino acids into flagella paralleled the rates of incorporation into the general cell protein. Mechanical removal of the flagella did not affect their subsequent rate of formation. The amino acid, ɛ--methyllysine, present in flagellin, can be labelled with either L-[-C]lysine or L-[-C]methionine. Using ɛ--methyl-lysine as a marker, it has not been possible to detect soluble protein precursors in the formation of flagella by

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1963-10-01
2021-10-24
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References

  1. Ambler R. P., Rees M. W. 1959; є- n -Methyllysine in bacterial flagellar protein.. Nature, Lond 186:86
    [Google Scholar]
  2. Bernstein A., Lederberg J. 1955; Agglutination of motile salmonellas by acridines.. J. Bact 69:142
    [Google Scholar]
  3. Davies R. 1959; Observations on the use of ultrasound waves for the disruption of micro-organisms.. Biochim. biophys. Acta 33:481
    [Google Scholar]
  4. Hughes D. E. 1951; A press for disrupting bacteria and other micro-organisms.. Brit. J. exp. Path 32:97
    [Google Scholar]
  5. Kauffmann F. 1954 Enterobacteriaceae Copenhagen: Ejnar Munksgaard;
    [Google Scholar]
  6. Kerridge D. 1959; Synthesis of flagella by amino acid-requiring mutants of Salmonella typhimurium. . J. gen. Microbiol 21:168
    [Google Scholar]
  7. Kerridge D. 1961a; The effect of inhibitors on the formation of flagella by Salmonella typhimurium. . J. gen. Microbiol 23:519
    [Google Scholar]
  8. Kerridge D. 1961b; Formation of bacterial flagella. Abstract of communication presented at the 5th int. Congr. Biochem. Moscow.
  9. Kerridge D., Horne R. W., Glauert A. M. 1962; Structural components of flagella from Salmonella typhimurium. . J. molec. Biol 4:227
    [Google Scholar]
  10. Ouchterlony O. 1953; Antigen-antibody reactions in gels. IV. Types of reaction in coordinated systems of diffusion.. Acta path. microbiol. scand 32:231
    [Google Scholar]
  11. Quadling C., Stocker B. A. D. 1956; An environmentally induced transition from the flagellated to the nonflagellated state in Salmonella: the fate of the parental flagella at cell division.. J. gen. Microbiol 15:i
    [Google Scholar]
  12. Quadling C., Stocker B. A. D. 1962; An environmentally induced transition from the flagellated to the non-flagellated state in Salmonella typhimurium: the fate of the parental flagella at cell division.. J. gen. Microbiol 28:257
    [Google Scholar]
  13. Roberts R. B., Cowie D. B., Abelson P. H., Bolton E. T., Britten R. J. 1955; Studies of biosynthesis in Escherichia coli. . Publ. Carneg. Instn607
    [Google Scholar]
  14. Sertic V., Boulgakov N. A. 1936; L'agglutination par la tryptoflavine des Salmonella de structure antigénique flagellaire non-spécifique.. C.R. Soc. biol., Paris 123:951
    [Google Scholar]
  15. Stocker B. A. D., Campbell J. C. 1959; The effect of non-lethal deflagellation on bacterial motility and observations on flagellar regeneration.. J. gen. Microbiol 20:670
    [Google Scholar]
  16. Stocker B. A. D., McDonough M. W., Ambler R. P. 1961; A gene determining presence or absence of є- n -methyllysine in Salmonella flagellar protein.. Nature, Lond 189:556
    [Google Scholar]
  17. Weinstein D., Koffler H., Moskowitz M. 1960; Evidence for the intracellular occurrence of flagellar proteins.. Bact. Proc p 60
    [Google Scholar]
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