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Abstract
SUMMARY: The influence of size of inoculum on the nitrogen metabolism of Aspergillus oryzae in submerged culture was studied. In producing a given weight of mycelium a large-inoculum culture, as compared with a small-inoculum culture, showed a higher degree of assimilation of nitrogen and a lower excretion of nitrogen over most of the growth curve. This comparatively greater efficiency of the large-inoculum culture became more pronounced when mechanical stress in the culture was increased. Under high mechanical stress, the inefficiency of a small-inoculum culture was so marked that in forming unit weight of mycelium it utilized more inorganic nitrogen than did a large-inoculum culture.
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© Journal of General Microbiology 1963