1887

Abstract

SUMMARY: Extracts made with buffered sodium diethyldithiocarbamate (DIECA) or potassium ethylxanthate from tobacco leaves infected with cucumber mosaic virus (CMV) were 5--500 times more infective than those made in buffer alone, or in buffer containing three other metal chelators which did not prevent the extracts going brown. DIECA preserved infectivity slightly better than did potassium ethylxanthate; both prevented browning equally. With DIECA in the extraction fluid, infectivity was not increased by other substances that enabled mitochondrial enzyme systems to be removed intact. CMV was inactivated by leaf polyphenols only when these were being oxidized. Oxidized polyphenols from virus-free leaves did not inactivate CMV when added together with copper, whereas deproteinized extracts of leaves crushed in an atmosphere of nitrogen did. When copper and chlorogenic acid, the main polyphenol in tobacco leaves, were added to infective extracts that contained polyphenoloxidase, the chlorogenic acid was oxidized and CMV was inactivated. A tobacco necrosis virus was slightly inactivated by incubating leaf extracts with chlorogenic acid and copper, but four other viruses were not. The tobacco necrosis virus was also the only one of these five to be at all stabilized by DIECA in the extraction fluid. The reported instability of some other plant viruses in leaf sap may mean that they also are susceptible to inactivation by polyphenoloxidase systems.

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1963-09-01
2024-12-09
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References

  1. Albert A., Rubbo S. D., Goldacre R. J., Balfour B. G. 1947; The influence of chemical constitution on antibacterial activity. Part III. A study of 8-hydroxy- quinoline (oxine) and related compounds. Brit. J. exp. Path 28:69
    [Google Scholar]
  2. Babos P., Kassanis B. 1962; Unstable variants of tobacco necrosis virus. Virology 18:206
    [Google Scholar]
  3. Barton G. M., Evans R. S., Gardner J. A. F. 1952; Paper chromatography of phenolic substances. Nature, Lond 170:249
    [Google Scholar]
  4. Bawden F. C., Pirie N. W. 1957; A virus-inactivating system from tobacco leaves. J. gen. Microbiol 16:696
    [Google Scholar]
  5. Bawden F. C., Pirie N. W. 1959; The infectivity and inactivation of nucleic acid preparations from tobacco mosaic virus. J. gen. Microbiol 21:438
    [Google Scholar]
  6. Best R. J., Samuel G. 1936; The effect of various chemical treatments on the activity of the viruses of tomato spotted wilt and tobacco mosaic. Ann. appl. Biol 23:759
    [Google Scholar]
  7. Bhargava K. S. 1951; Some properties of four strains of cucumber mosaic virus. Ann. appl. Biol 38:377
    [Google Scholar]
  8. Bonner J., Wildman S. G. 1946; Enzymatic mechanisms in the respiration of spinach leaves. Arch. Biochem. Biophys 10:497
    [Google Scholar]
  9. Cadman C. H. 1959; Some properties of an inhibitor of virus infection from leaves of raspberry. J. gen. Microbiol 20:113
    [Google Scholar]
  10. Cadman C. H. 1962; Evidence for association of tobacco rattle virus nucleic acid with a cell component. Nature, Lond 193:49
    [Google Scholar]
  11. Dawson C. R., Tarpley W. B. 1951; Copper oxidases. In The Enzymes vol ii part 1, p 454 Edited by Sumner J. B., Myrbäck K. New York: Academic Press;
    [Google Scholar]
  12. Dias H. F. 1962 Sap-transmissible viruses of grapevines Ph.D. Thesis: University of London;
    [Google Scholar]
  13. Frey-Wyssling A., Babler S. 1957; Zur Biochemie des Gewächshaustabaks. Experientia 13:399
    [Google Scholar]
  14. Fulton R. W. 1949; Virus concentration in plants acquiring tolerance to tobacco streak. Phytopathology 39:231
    [Google Scholar]
  15. Fulton R. W. 1952; Mechanical transmission and properties of rose mosaic virus. Phytopathology 42:413
    [Google Scholar]
  16. Hampton R. E., Fulton R. W. 1961; The relation of polyphenol oxidase to instability in vitro of prune dwarf and sour cherry necrotic ringspot viruses. Virology 13:44
    [Google Scholar]
  17. Hill J. M., Mann P. J. G. 1962; The inhibition of pea-seedling diamine oxidase by chelating agents. Biochem. J 85:198
    [Google Scholar]
  18. Hillis W. E., Carle A. 1962; The origin of the wood and bark polyphenols of Eucalyptus species. Biochem. J 82:435
    [Google Scholar]
  19. Hulme A. C. 1953; The isolation of chlorogenic acid from the apple fruit. Biochem. J 53:337
    [Google Scholar]
  20. Jones J. D., Hulme A. C. 1961; Preparation of mitochondria from the peel of apples. Nature, Lond 191:370
    [Google Scholar]
  21. Kassanis B., Nixon H. L. 1961; Activation of one tobacco necrosis virus by another. J. gen. Microbiol 25:459
    [Google Scholar]
  22. Mann P. J. P. 1955; Purification and properties of the amine oxidase of pea seedlings. Biochem. J 59:609
    [Google Scholar]
  23. Mink G. I., Bancroft J. B. 1962; Purification and serology of Tulare apple mosaic virus. Nature, Lond 194:214
    [Google Scholar]
  24. Pierpoint W. S. 1959; Mitochondrial preparations from the leaves of tobacco (Nicotiana tabacwn). Biochem. J 71:518
    [Google Scholar]
  25. Pirie N. W. 1961; The disintegration of soft tissues in the absence of air. J. agric. Engng Res 6:142
    [Google Scholar]
  26. Roberts E. A. H., Wood D. J. 1951; The polyphenols and amino acids of tobacco leaf. Arch. Biochem. Biophys 33:299
    [Google Scholar]
  27. Sänger H. L., Brandenburg E. 1961; Über die Gewinnung von infektiösem Press-saft aus ‘Wintertyp’ — Pflanzen des Tabak-Rattle-Virus durch Phenolextraktion. Naturwissenschaften 48:391
    [Google Scholar]
  28. Schlegel D. E. 1960; Highly infectious phenol extracts from tobacco leaves infected with cucumber mosaic virus. Virology 11:329
    [Google Scholar]
  29. Shiroya M., Shiroya T., Hattori S. 1955; Studies on the browning and blackening of plant tissues. IV. Chlorogenic acid in the leaves of Nicotiana tabacum.. Physiol. Plant 8:594
    [Google Scholar]
  30. Weaving A. S. 1958; Methods of determining chlorogenic acid in tobacco. Chem. & Ind p 216
    [Google Scholar]
  31. Yarwood C. E. 1955; Mechanical transmission of an apple mosaic virus. Hilgardia 23:613
    [Google Scholar]
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