1887

Abstract

SUMMARY: Antibiotic production in liquid media by strains of bacteriophage ‘type 71’ was poor as measured by cup assays against susceptible corynebacteria. Aerobic incubation in freshly prepared tryptic-digest broth containing a fermentable carbohydrate and adjusted to pH 7.8 gave the greatest yields. The antibiotic material was not obtained in a pure state, but was concentrated by evaporation of crude solutions. These were prepared by adding trichloroacetic acid to broth cultures and centrifuging to remove the organisms. The antibiotic was stable and relatively heat-resistant under acid conditions, but was rapidly destroyed when alkaline; it diffused slowly through cellophan, was adsorbed by charcoal, and was inactivated by trypsin but not by pepsin. These properties suggest that it may be protein or polypeptide in nature. Inhibitory activity by type 71 cocci was shown in mixed broth cultures against other non-inhibitory strains of and was similar to that previously observed on solid media.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-32-2-255
1963-08-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/32/2/mic-32-2-255.html?itemId=/content/journal/micro/10.1099/00221287-32-2-255&mimeType=html&fmt=ahah

References

  1. Barrow G. I. 1955; Clinical and bacteriological aspects of impetigo contagiosa. J. Hyg., Camb 53:495
    [Google Scholar]
  2. Barrow G. I. 1963; Microbial antagonism by Staphylococcus aureus. J. gen. Microbiol 31:471
    [Google Scholar]
  3. Fredericq P. 1957; Colicins. Annu. Rev. Microbiol 11:7
    [Google Scholar]
  4. Gardner J. F. 1949; An antibiotic produced by Staphylococcus aureus. Brit. J. exp. Path 30:130
    [Google Scholar]
  5. Halbert S. P., Swick L., Sonn C. 1953; Characteristics of antibiotic-producing strains of the ocular bacterial flora. J. Immunol 70:400
    [Google Scholar]
  6. Loeb L. J., Moyer A., Murray R. G. E. 1950; An antibiotic produced by Micrococcus epidermidis. Canad. J. Res (E) 28:212
    [Google Scholar]
  7. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb 38:732
    [Google Scholar]
  8. Parker M. T. 1958; Some cultural characteristics of Staphylococcus aureus strains from superficial skin infections. J. Hyg., Camb 56:238
    [Google Scholar]
  9. Parker M. T., Simmons L. E. 1959; The inhibition of Corynebacterium diphtheriae and of other Gram-positive organisms by Staphylococcus aureus. J. gen. Microbiol 21:457
    [Google Scholar]
  10. Parker M. T., Tomlinson A. J. H., Williams R. E. O. 1955; Impetigo contagiosa. The association of certain types of Staphylococcus aureus and of Streptococcus pyogenes with superficial skin infections. J. Hyg., Camb 53:458
    [Google Scholar]
  11. Waksman S. A. 1947 Microbial antagonisms and antibiotic substances 2nd ed New York: Commonwealth Fund;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-32-2-255
Loading
/content/journal/micro/10.1099/00221287-32-2-255
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error