1887

Abstract

SUMMARY: A study of 22 haemagglutinating viruses was made to see whether by treating the viruses and the receptors on the red cells with a variety of physical and chemical agents, a convenient means could be devised for identifying and classifying these viruses, while throwing some light on the chemical basis of the haemagglutination reaction. The viruses were submitted to 13 different treatments; acid, urea, -chloromercuriben-zoic acid, deoxycholate and possibly bisulphite might be useful for the classification of unknown agents since they gave similar results with all members of a biological group. Other treatments (e.g. formaldehyde) gave results which varied from strain to strain in such a group of viruses and might be useful for genetic studies. The red cells were treated in nine different ways; formalin, papain, chymotrypsin, periodate, receptor-destroying enzyme, (RDE) and swine influenza virus each prevented agglutination by one or two viruses (apparently by inactivating cell receptors). These results were complementary to those with the virus haemagglutinins. The importance of standardized conditions of test are emphasized.

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/content/journal/micro/10.1099/00221287-32-2-241
1963-08-01
2021-10-18
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References

  1. Andrewes C. H., Bang F. B., Burnet F. M. 1955; A short description of the myxo-virus group (influenza and related viruses). Virology 1:176
    [Google Scholar]
  2. Buckland F. E. 1959; Impairment of viral haemagglutination of red cells after treatment with formalin. Nature, Lond 183:1276
    [Google Scholar]
  3. Buckland F. E. 1960; Inactivation of virus haemagglutinins by para-chloromercuri-benzoic acid. Nature, Lond 188:768
    [Google Scholar]
  4. Burnet F. M., Stone Joyce D. 1947; The receptor destroying enzyme of V. cholerae. Aust. J. exp. Biol. med. Sci 25:227
    [Google Scholar]
  5. Cheng P.Y. 1961; Some physical properties of haemagglutinating and complementfixing particles of Semliki Forest virus. Virology 14:132
    [Google Scholar]
  6. Clarke D., Casals J. 1958; Techniques for haemagglutination and haemagglutination inhibition with arthropod-borne viruses. Amer. J. trop. Med. Hyg 7:561
    [Google Scholar]
  7. Choppin P. W., Tamm I. 1960; Studies of two kinds of virus particles which comprise A2 virus strains. J. exp. Med 112:895
    [Google Scholar]
  8. Fazekas de St Groth S. 1949; Modification of virus receptors by metaperiodate. I. The properties of I04-treated cells. Aust. J. exp. Biol. med. Sci 27:65
    [Google Scholar]
  9. Goldfield M., Srihongse S., Fox J. P. 1957; Haemagglutinins associated with certain human enteric viruses. Proc. Soc. exp. Biol., N.Y 96:788
    [Google Scholar]
  10. Gresser I., Enders J. F. 1961; The effect of trypsin on representative myxoviruses. Virology 13:420
    [Google Scholar]
  11. Hirst G. K. 1948; The nature of virus receptors of red cells. I. Evidence on the chemical nature of the virus receptors of red cells and of the existence of a closely analogous substance in normal serum. J. exp. Med 87:301
    [Google Scholar]
  12. Kasel J. A., Rowe W. P., Nemes J. L. 1960; Modification of erythrocyte receptors by a factor in adenovirus suspension. Virology 10:388
    [Google Scholar]
  13. Martin E. M., Malec J., Sved S., Work T. S. 1961; Studies on protein and nucleic acid metabolism in virus infected mammalian cells. Part I. Assessment of a suitable system. Biochem. J 80:585
    [Google Scholar]
  14. Muschel L. H., Piper D. R. 1959; Enzyme treated red blood cells of sheep in the test for infectious mononucleosis. Amer. J. din. Path 32:240
    [Google Scholar]
  15. Philipson L. 1959; Studies on the mechanism of haemagglutination by Echo viruses. I. Evidence for enzymatic degradation by chymotrypsin of the receptor for Echo viruses. Arch. ges. Virusforsch 9:251
    [Google Scholar]
  16. Philipson L., Choppin P. W. 1960; On the role of virus sulphydryl groups in the attachment of enteroviruses to erythrocytes. J. exp. Med 112:455
    [Google Scholar]
  17. Takatsy G., Barb K. 1959; New inhibitor and erythrocyte receptor substances for certain Asian strains of influenza virus. Nature, Lond 183:52
    [Google Scholar]
  18. Theiler M. 1957; Action of sodium deoxycholate on arthropod-borne viruses. Proc. Soc. exp. Biol., N.Y 96:380
    [Google Scholar]
  19. Tyrrell D. A. J., Buckland F. E., Lancaster M. C., Valentine R. C. 1960; Some properties of a strain of SV 17 virus isolated from an epidemic of conjunctivitis and rhinorrhea in monkeys (Erythrocebus patas). Brit. J. exp. Path 41:610
    [Google Scholar]
  20. Underwood G. E., Weed S. D. 1961; The effect of carbonyl compounds on poliovirus. Virology 13:138
    [Google Scholar]
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