1887

Abstract

SUMMARY: Several steroids closely related structurally to cholesterol were tested for growth-promoting activity for a strain of (V5) and a sp. isolated from a goat (). Cholestanol and latho-sterol promoted growth of both strains. Cholestenone, cholest-5-en-3-one, 7-dehydrocholesterol and progesterone inhibited cholesterol-promoted growth; cholestanone was almost inactive either as growth promotor or as growth inhibitor. Cholesterol amounted to 4–5% of the dry weight of the water-washed organisms, or about 20% of the total lipids, of either strain grown in the presence of cholesterol. Cholesterol esters or transformations to other steroids were not detected in lipid extracts of the v5 strain grown in the presence of cholesterol-4-C. The strain was grown in the presence of cholesterol-4-C of known specific activity, the lipid extract diluted with a known amount of unlabelled cholesterol, and cholesterol isolated and purified. Its specific activity was very close to the value expected if no sterol transformations had occurred. When grown in the presence of cholestanol, the strain incorporated this sterol without desaturation.

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1963-07-01
2024-12-09
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References

  1. Barton D. H. R., Cox J. D. 1948; The application of the method of molecular structure and action differences in steroids. Part IV. Optical anomalies. J. chem. Soc p 783
    [Google Scholar]
  2. Butler M., Knight B. C. J. G. 1960; Steroid growth requirements and steroid growth inhibitors of Mycoplasma. J. gen. Microbiol 22:483
    [Google Scholar]
  3. Edward D. G. FF., Fitzgerald W. A. 1951; Cholesterol in the growth of organisms of the pleuropneumonia group. J. gen. Microbiol 5:576
    [Google Scholar]
  4. Fieser L. F. 1953; Cholesterol and companions. VII. Steroid dibromides. J. Amer. chem. Soc 75:5421
    [Google Scholar]
  5. Folch J., Lees M., & Sloane- Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem 226:497
    [Google Scholar]
  6. Kabara J. J., McLaughlin J. T. 1961; A microdibromide procedure for purifying radioactive cholesterol. J. lipid Res 2:283
    [Google Scholar]
  7. Kodicek E., Ashby D. R. 1954; Paper chromatography of vitamin D and other sterols. Biochem. J 57:xii
    [Google Scholar]
  8. Laws L. 1956; A pleuropneumonia-like organism causing peritonitis in goats. Aust. vet. J 32:326
    [Google Scholar]
  9. Lynn R. J., Smith P. F. 1960; Chemical composition of PPLO. Ann. N.Y. Acad. Sci 79:493
    [Google Scholar]
  10. Martin R. P. 1957; Reversed phase paper chromatography and detection of steroids of the cholesterol class. Biochim. biophys. Acta 25:408
    [Google Scholar]
  11. Martin R. P., Bush I. E. 1955; Application of a reversed phase system for separation of the less polar steroids on paper. Fed. Proc 14:252
    [Google Scholar]
  12. Michalec C., Strasek J. 1960; A new paper chromatographic technique for the separation of cholesterol and cholesteryl esters. J. Chromatog 4:254
    [Google Scholar]
  13. Morowitz H. J., Tourtellotte M. E., Guild W. R., Castro, Elisea, Woess C., Cleverdon R. C. 1962; The chemical composition and submicroscopic morphology of Mycoplasma gallisepticum Avian PPLO 5969. J. molec. Biol 4:93
    [Google Scholar]
  14. Page I. H., Rudy H. 1930; Über die Fettsäureester des Cholesterins. Biochem. Z 220:304
    [Google Scholar]
  15. Peereboom J. W., Copius J. W., Roos J. B. 1960; Fette, Seifen, Ausstrichmittel. 62, 91. Cited. in J. Chromatog (1961) 5:D15
    [Google Scholar]
  16. Rodwell A. W. 1956; The role of serum in the nutrition of. Asterococcus mycoides. Aust. J. biol. Sci 9:105
    [Google Scholar]
  17. Rodwell A. W. 1960; Nutrition and metabolism of Mycoplasma mycoides var. mycoides. Ann. N.Y. Acad. Sci 79:499
    [Google Scholar]
  18. Rodwell A. W., Abbot A. 1961; The function of glycerol, cholesterol and long-chain fatty acids in the nutrition of. Mycoplasma mycoides. J. gen. Microbiol 25:201
    [Google Scholar]
  19. Rothblat G. H., Smith P. F. 1961; Non-saponifiable lipids of representative PPLO. J. Bact 82:479
    [Google Scholar]
  20. Smith P. F. 1959; Cholesterol esterase activity of pleuropneumonia-like organisms. J. Bact 77:682
    [Google Scholar]
  21. Smith P. F. 1960; Nutritional requirements of PPLO and their relation to metabolic function. Ann. N.Y. Acad. Sci 79:508
    [Google Scholar]
  22. Smith P. F. 1962; Fate of ergosterol and cholestanol in pleuropneumonia-like organisms. J. Bad 84:534
    [Google Scholar]
  23. Smith P. F., Lynn R. J. 1958; Lipid requirements for the growth of pleuropneumonia-like organisms. J. Bact 76:264
    [Google Scholar]
  24. Sperry W. M., Webb M. 1950; A revision of the Schoenheimer–Sperry method for cholesterol determination. J. biol. Chem 187:97
    [Google Scholar]
  25. Swell L., Treadwell C. R. 1955; Cholesterol esterases. VI. Relative specificity and activity of pancreatic cholesterol esterase. J. biol. Chem 212:141
    [Google Scholar]
  26. Turner A. W., Campbell A. D., Dick A. T. 1935; Recent work on pleuropneumonia Contagiosa boum in North Queensland. Aust. vet. J 11:63
    [Google Scholar]
  27. Vahouny G. V., Mayer R. M., Roe J. H., Treadwell C. R. 1960; Determination of 3β-hydroxy sterols with anthrone reagent. Arch. Biochem. Biophys 86:210
    [Google Scholar]
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