1887

Abstract

SUMMARY:

Freeze-dried spores of and type E suffered little or no loss in viability after storage at 25° at water activity () values between 0.2 and 0.8. When stored over PO (0.00 ) the spores of all four species showed a marked loss in viability. The above results were similar for spores whether stored in air or in vacuum. With spores stored over distilled water (1.00 ) the Bacillus spores underwent a large loss of viability in vacuum, but not in air; for spores of the clostridia the reverse was true. The addition of DL-glyceraldehyde, diacetyl or ribose (0.05 M) to the spore suspensions before drying caused increased death during storage at 0.50 and to a lesser extent at 0.20 Death was greater at 30° than at 10°. The addition of sucrose, glutamate or semi-carbazide did not decrease the viability. When the dried spores were resuspended in dilute phosphate buffer after storage for 2.6 years their resistance to heating was greatest after storage at values of 0.4, 0.6 and 0.8.

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/content/journal/micro/10.1099/00221287-31-3-451
1963-06-01
2024-04-19
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References

  1. Asheshov I. N. 1941; Papain digest media and standardisation of media in general. Canad. J. publ. Hlth 32:468
    [Google Scholar]
  2. Brewer J. H. 1940; Clear liquid medium for the ‘aerobic’ cultivation of anaerobes. J. Amer. med. Ass. 115:598
    [Google Scholar]
  3. Bullock K., Lightbown J. W. 1947; The inactivation of enzymes and microorganisms in oils and powders. Part II. The effects of drying on the viability of bacteria, and their thermolability in powders. Quart. J. Pharm. 20:312
    [Google Scholar]
  4. Bullock K., Tallentire A. 1952; Bacterial survival in systems of low moisture content. Part IV. The effects of increasing moisture content on heat resistance, viability and growth of spores of B. subtilis. J. Pharm., Lond. 4:917
    [Google Scholar]
  5. Dubovsky B. J., Meyer K. F. 1922; An experimental study of the methods available for the enrichment, demonstration, and isolation of B. botulinus in specimens of soil and its products, in suspected food, in clinical and necropsy material. J. infect. Dis. 31:501
    [Google Scholar]
  6. Evans F. R., Curran H. R. 1960; Influence of preheating, pH, and holding temperatures upon viability of bacterial spores stored for long periods in buffer substrates. J. Bact. 79:361
    [Google Scholar]
  7. Hawrylewicz E., Gowdy B., Ehrlich R. 1962; Micro-organisms under a simulated Martian environment. Nature, Lond. 193:497
    [Google Scholar]
  8. Leach R. H., Scott W. J. 1959; The influence of rehydration on the viability of dried micro-organisms. J. gen. Microbiol. 21:295
    [Google Scholar]
  9. Lion M. B., Bergmann E. D. 1961; The effects of oxygen on freeze-dried Escherichia coli.. J. gen. Microbiol. 24:191
    [Google Scholar]
  10. Magoon C. A. 1926; Studies upon bacterial spores. I. Thermal resistance as affected by age and environment. J. Bact. 11:253
    [Google Scholar]
  11. Murrell W. G., Scott W. J. 1957; Heat resistance of bacterial spores at various water activities. Nature, Lond. 179:481
    [Google Scholar]
  12. Ohye D. F., Murrell W. G. 1962; Formation and structure of the spore of Bacillus coagulans. J. Cell Biol. 14:111
    [Google Scholar]
  13. Robinow C. F. 1951; Observations on the structure of Bacillus spores. J. gen. Microbiol. 5:439
    [Google Scholar]
  14. Robinson R. A., Stokes R. H. 1955 Electrolyte Solutions. Appendix 8.11 490 London: Butterworths;
  15. Scott W. J. 1958; The effect of residual water on the survival of dried bacteria during storage. J. gen. Microbiol. 19:624
    [Google Scholar]
  16. Scott W. J. 1960 A mechanism causing death during storage of dried micro-organisms. Recent Research in Freezing and Drying188 Edited by Parkes A. S., Smith A. U. Oxford: Blackwell;
    [Google Scholar]
  17. Tallentire A. 1958; An observed Oxygen effect’ during gamma-irradiation of dried bacterial spores. Nature, Lond. 182:1024
    [Google Scholar]
  18. Williams O. B. 1929; Heat resistance of bacterial spores. J. infect. Dis. 44:421
    [Google Scholar]
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