1887

Abstract

SUMMARY:

Spontaneous mutations, and those induced by nitrous acid or ultraviolet light, were studied in the haploid fission yeast Reverse mutations conferring ability to grow on a minimal medium lacking adenine were scored in adenine auxotrophs, and in di-auxotrophs having requirements for both adenine and methionine. The apparently lower mutability of the mutants in the diauxotrophic strains compared with the strains was shown to be due to the influence of methionine in the plating medium. L-methionine added to the minimal medium at a concentration of 40 μg. per ml. will suppress the appearance of spontaneous and induced revertants of mutants. A similar effect has been shown for reversions of an adenine mutant at another locus, and for reversions of a leucine auxotroph. Methionine has the effect of decreasing the extent of residual divisions undergone by adenine-requiring cells when plated on a minimal medium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-31-3-353
1963-06-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/31/3/mic-31-3-353.html?itemId=/content/journal/micro/10.1099/00221287-31-3-353&mimeType=html&fmt=ahah

References

  1. Auerbach C., Kølmark G. 1960; Mutagen specificity and combination treatments in Neurospora. Microbiol. Gen. Bull. 17:23
    [Google Scholar]
  2. Bertani G. 1951; A method for detection of mutations using streptomycin dependence in Escherichia coli.. Genetics 36:598
    [Google Scholar]
  3. Clarke C. H. 1962; A case of mutagen specificity attributable to a plating medium effect. Z. Vererb.-Lehre 93:435
    [Google Scholar]
  4. Clavilier L., Luzzati M., Slonimski P. P. 1960; Sur la conversion du gène ad 3 chez la levure. C.B. Soc. Biol., Paris 154:1970
    [Google Scholar]
  5. Fritz-Niggli H. 1960; Röntgenstrahleninduzierte Rückmutationen (Punktmutationen) bei Schizosaccharomyces pombe in Abhängigkeit vom Zeilzustand, Bestrahlungsmilieu und Agens der Vorwärtsinduktion. Naturwissenschaften 18:429
    [Google Scholar]
  6. Giese A. C, Iverson R. M., Sanders R. T. 1957; Effect of nutritional state and other conditions on ultra-violet resistance and photoreactivation in yeast. J. Bact. 74:271
    [Google Scholar]
  7. Glover S. W. 1956; A comparative study of induced reversions in Escherichia coli.. Publ. Carneg. Instn 612:121
    [Google Scholar]
  8. Grigg G. W. 1952; Back mutation assay in micro-organisms. Nature, Lond. 169:98
    [Google Scholar]
  9. Heslot H. 1960; Schizosaccharomyces pombe’. un nouvel organisme pour l’étude de la mutagenèse chimique. Abh. dtsch. Akad. Wiss. Berl., Klasse Medizin 1:98
    [Google Scholar]
  10. Heslot H. 1962; Etude quantitative de reversions biochimique induites chez la levure Schizosaccharomyces pombe par des radiations et des substances radiomimetiques. Abh. dtsch. Akad. Wiss. Berl., Klasse Medizin 1:193
    [Google Scholar]
  11. Leupold U. 1955; Versuche zur genetischen Klassifizierung adeninabhängiger Mutanten von Schizosaccharomyces pombe. Arch. Klaus-Stift. VererbForsch. 30:506
    [Google Scholar]
  12. Leupold U. 1958; Studies on recombination in Schizosaccharomyces pombe. Cold Spr. Horb. Symp. quant. Biol. 23:161
    [Google Scholar]
  13. Leupold U. 1961; Intragene Rekombination und allele Komplementierung. Arch. Klaus-Stift. VererbForsch. 36:89
    [Google Scholar]
  14. Lindegren C. C. 1949 The Y east Cell: Its Genetics and Cytology Chap. 15 St Louis: Educational Publishers Inc;
  15. Luzzati M., Claviller L., Slonimski P. P. 1959; Inhibition par l’histidine de la réversion prototrophe chez la levure diploide hétéroallélique pour le locus adénine-histidine. CR. Acad. Sci. Pans 249:1412
    [Google Scholar]
  16. Miyake T. 1960; Mutator factor in Salmonella typhimurium. Genetics 45:11
    [Google Scholar]
  17. Reader V. 1927; The relation of the growth of certain micro-organisms to the composition of the medium. I. The synthetic culture medium. Biochem. J. 21:901
    [Google Scholar]
  18. Rudner R. 1961; Mutation as an error in base pairing. I. The mutagenicity of base analogues and their incorporation into the DNA of Salmonella typhimurium. Z. Vererb.-Lehre 92:336
    [Google Scholar]
  19. Treffers H. P., Spinelli V., Belser N. O. 1954; A factor (or mutator gene) influencing mutation rates in Escherichia coli.. Proc. nat. Acad. Sci., Wash. 40:1064
    [Google Scholar]
  20. Witkin E. M., Theil E. C. 1960; The effect of post-treatment with chloramphenicol on various ultraviolet induced mutations in Escherichia coli.. Proc. nat. Acad. Sci., Wash. 46:226
    [Google Scholar]
  21. Woodward J. D., Rainbow C. 1961; 5-aininoimidazole and its riboside from biotin-deficient yeast. J. gen. Microbiol. 25:141
    [Google Scholar]
  22. Zetterberg G. 1960; The mutagenic effect of 8-ethoxycaffein caffein and dimethyl-sulphate in the Ophiostoma back-mutation test. Hereditas 46:279
    [Google Scholar]
  23. Zetterberg G. 1962; Genetic influence on the back-mutation rate in biochemical mutant strains of Ophiostoma. Exp. Cell Res. 27:560
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-31-3-353
Loading
/content/journal/micro/10.1099/00221287-31-3-353
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error